Source Paper
Sex-dependent effects of ultra-low-dose-THC preventive treatment on neuroinflammation and cognitive decline in 5xFAD mice
Nitzan K, Bentulila Z, Bregman-Yemini N, Ayalon N, David D et al.
Biol Sex Differ • 2026
Y-Maze
Objective: Evaluate short-term working memory (PFC-dependent task)
This is a Y-Maze protocol using mice as the model organism. The procedure involves 3 procedural steps, 3 equipment items, 1 materials. Extracted from a 2026 paper published in Biol Sex Differ.
Model and subjects
mice • 5xFAD mouse model and WT littermates • male and female • 6 months • 80
Study window
Estimated timing pending
Core workflow
Mouse placement • Recording arm entries • Arena cleaning
Primary readouts
- Correct triads - triads with all three arms represented (i.e., ABC, CAB, or BCA but not ABB)
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
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- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
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Mouse placement
Place mice initially in the middle of the Y-maze
View evidence from paper
“Mice were initially placed in the middle”
Recording arm entries
Record the sequence of arm entries for each mouse
View evidence from paper
“the sequence of arm entries was recorded for each mouse over an 8-min period”
Arena cleaning
Clean the Y-maze arena with alcohol between mice to eliminate olfactory cues
View evidence from paper
“The arenas of the Y-maze, OFH and PLT were cleaned with Alcohol between mice, in order to eliminate olfactory cues”