Source Paper
The potential of human milk oligosaccharides in ameliorating traumatic brain injury-induced cognitive impairment in mice
He D, He R, Duan W, Li G, Kang Q
Future Sci OA • 2026
Y-Maze Test
behavioralmiceC57BL/6
Objective: Assessment of spatial working memory through spontaneous alternation behavior in mice
Materials & Equipment Checklist
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Protocol Steps
1
Test timing
Conduct Y-maze test on the 16th day post-TBI
not specified
View evidence from paper
“The YM test was conducted on the 16th day post-TBI”
2
Arm designation
Randomly designate one arm as the 'start arm'
not specified
View evidence from paper
“One arm was randomly designated as the 'start arm'”
3
Mouse placement and exploration
Place mouse in the start arm and allow free exploration of the maze
5 minutes
View evidence from paper
“the mouse was placed therein, with free exploration of the maze permitted for 5 minutes”
4
Behavioral recording
Record entries into each arm (Arms A–C) using Ethovision XT software
during 5-minute exploration
View evidence from paper
“The behavioral activity of the mice was analyzed using Ethovision XT software, and entries into each arm (Arms A–C) were recorded”