Source Paper
The potential of human milk oligosaccharides in ameliorating traumatic brain injury-induced cognitive impairment in mice
He D, He R, Duan W, Li G, Kang Q
Future Sci OA • 2026
Y-Maze Test
Objective: Assessment of spatial working memory through spontaneous alternation behavior in mice
This is a Y-Maze Test protocol using mice as the model organism. The procedure involves 4 procedural steps, 1 equipment items, 1 materials. Extracted from a 2026 paper published in Future Sci OA.
Model and subjects
mice • C57BL/6 • not specified • 6–8 weeks old • 48
Study window
~5 minutes hands-on
Core workflow
Test timing • Arm designation • Mouse placement and exploration
Primary readouts
- Arm entries (counted when all four paws of the mouse entered the arm)
- Total alternations (consecutive entries into three distinct arms)
- Spontaneous alteration percentage (calculated as: (total alternations × 100)/(total arm entries − 2))
- Spatial working memory integrity (spontaneous alteration exceeding 50%)
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Test timing
Conduct Y-maze test on the 16th day post-TBI
View evidence from paper
“The YM test was conducted on the 16th day post-TBI”
Arm designation
Randomly designate one arm as the 'start arm'
View evidence from paper
“One arm was randomly designated as the 'start arm'”
Mouse placement and exploration
Place mouse in the start arm and allow free exploration of the maze
View evidence from paper
“the mouse was placed therein, with free exploration of the maze permitted for 5 minutes”
Behavioral recording
Record entries into each arm (Arms A–C) using Ethovision XT software
View evidence from paper
“The behavioral activity of the mice was analyzed using Ethovision XT software, and entries into each arm (Arms A–C) were recorded”