Source Paper
Ablation of Unilateral Hippocampal GABAergic Neurons: A Novel Mouse Model of Mesial Temporal Lobe Epilepsy With Hippocampal Sclerosis
Tang T, Xu J, Fu B, Geng C, Li Y et al.
CNS Neurosci Ther • 2026
Novel Object Recognition Test
Objective: To evaluate recognition memory in animal models within the inter-ictal periods in epilepsy models
Gather these items before starting the experiment. Check off items as you prepare.
1 item available from ConductScience
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Protocol Steps
Day 1 - Empty arena exploration
Allow mice to explore the empty arena for 10 minutes
View evidence from paper
“mice were allowed to explore the empty arena for 10 min on Day 1”
Day 2 - Familiarization phase
Place a single mouse in the arena with two identical objects placed symmetrically and allow free exploration for 10 minutes
View evidence from paper
“a single mouse was allowed to freely explore the arena with two identical objects (A1 and A2) symmetrically placed for 10 min”
Day 3 - Novel object recognition test
Replace one familiar object (A1) with a novel object (B) and record exploration time for each object during a 10-minute test
View evidence from paper
“the familiar object (A1) is replaced with a novel object (B), and exploration time for each object is recorded during 10‐min tests”
Define exploration behavior
Define exploration as sniffing, touching, or directing the nose toward the object within 2 cm or less
View evidence from paper
“Exploration is defined as sniffing, touching, or directing the nose toward the object within ≤ 2 cm”
Calculate discrimination index
Calculate the discrimination index using the formula: DI = (T novel - T familiar)/(T novel + T familiar)
View evidence from paper
“The discrimination index (DI) was calculated as DI = ( T novel − T familiar )/( T novel + T familiar )”