Source Paper
Ablation of Unilateral Hippocampal GABAergic Neurons: A Novel Mouse Model of Mesial Temporal Lobe Epilepsy With Hippocampal Sclerosis
Tang T, Xu J, Fu B, Geng C, Li Y et al.
CNS Neurosci Ther • 2026
Novel Object Recognition Test
Objective: To evaluate recognition memory in animal models within the inter-ictal periods in epilepsy models
This is a Novel Object Recognition Test protocol using Mouse as the model organism. The procedure involves 5 procedural steps, 1 equipment items, 3 materials. Extracted from a 2026 paper published in CNS Neurosci Ther.
Model and subjects
Mouse • C57BL/6 and VGAT-ires-Cre mice • Male • approximately 8 weeks old • Not specified
Study window
~20 minutes hands-on
Core workflow
Day 1 - Empty arena exploration • Day 2 - Familiarization phase • Day 3 - Novel object recognition test
Primary readouts
- Exploration time for novel object
- Exploration time for familiar object
- Discrimination index (DI)
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Day 1 - Empty arena exploration
Allow mice to explore the empty arena for 10 minutes
View evidence from paper
“mice were allowed to explore the empty arena for 10 min on Day 1”
Day 2 - Familiarization phase
Place a single mouse in the arena with two identical objects placed symmetrically and allow free exploration for 10 minutes
View evidence from paper
“a single mouse was allowed to freely explore the arena with two identical objects (A1 and A2) symmetrically placed for 10 min”
Day 3 - Novel object recognition test
Replace one familiar object (A1) with a novel object (B) and record exploration time for each object during a 10-minute test
View evidence from paper
“the familiar object (A1) is replaced with a novel object (B), and exploration time for each object is recorded during 10‐min tests”
Define exploration behavior
Define exploration as sniffing, touching, or directing the nose toward the object within 2 cm or less
View evidence from paper
“Exploration is defined as sniffing, touching, or directing the nose toward the object within ≤ 2 cm”
Calculate discrimination index
Calculate the discrimination index using the formula: DI = (T novel - T familiar)/(T novel + T familiar)
View evidence from paper
“The discrimination index (DI) was calculated as DI = ( T novel − T familiar )/( T novel + T familiar )”