Novelty Suppressed Feeding
Objective: Assess anxiety-like behavior through food consumption latency in a novel environment
Protocol Steps
Animal acclimation to testing room
Animals were given habituation time to the behavioral testing room prior to testing
Note: Performed between 0900h and 1600h
View evidence from paper
“Animals were given 30 min of habituation to the behavioral testing room. Behavioral phenotyping was performed between 0900 h and 1600 h.”
Baseline behavioral testing sequence
Tests performed in order from least to most invasive: rotarod, elevated plus maze, marble burying test, open field test, sucrose preference test, novelty suppressed feeding, and forced swim test
Note: Order reversed after chronic restraint stress for bell-shaped stress exposure
View evidence from paper
“Tests were performed from the least to the most invasive to minimize the influence of prior test history (in order: rotarod, elevated plus maze, marble burying test, open field test, sucrose preference test, novelty suppressed feeding and forced swim test)”
Novelty suppressed feeding test
Assessment of anxiety-like behavior through food consumption latency in a novel environment
Note: Specific procedural details for this test are referenced in Supplementary Materials and Methods but not detailed in main text
View evidence from paper
“novelty suppressed feeding”
Chronic restraint stress application
After baseline behavioral testing, animals were submitted to restraint stress for 21 days. Each day mice were placed in a horizontal resting position inside a well-ventilated 50 ml falcon tube
Note: Restraint applied at 1000h daily; mice unrestrained after 4-6 hours
View evidence from paper
“After baseline behavioral testing, animals were submitted to restraint stress for 21 days. Every day, mice were placed in a horizontal resting position inside a well-ventilated (12 holes, 0.5 mm diameter) 50 ml falcon tube at 1000 h and after 4-6 h they were unrestrained.”