Source Paper
Sex-dependent effects of ultra-low-dose-THC preventive treatment on neuroinflammation and cognitive decline in 5xFAD mice
Nitzan K, Bentulila Z, Bregman-Yemini N, Ayalon N, David D et al.
Biol Sex Differ • 2026
Open-Field Habituation
Objective: Evaluates long-term non-associative, non-aversive spatial learning by measuring the decrease in exploratory activity
This is a Open-Field Habituation protocol using Mouse as the model organism. The procedure involves 4 procedural steps, 3 equipment items, 1 materials. Extracted from a 2026 paper published in Biol Sex Differ.
Model and subjects
Mouse • 5xFAD and C57B/Rcc • Male and female • 6 months • 80
Study window
~1 day study window | ~10 minutes hands-on
Core workflow
First exposure session • Inter-session interval • Second exposure session
Primary readouts
- Distance walked in first session
- Distance walked in second session
- Delta between sessions (difference in distance walked)
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
First exposure session
Place animal in novel open field environment and measure distance walked
View evidence from paper
“animals were exposed to a novel environment by placing them in a 40 cm × 40 cm × 40 cm open field box. The distance walked was measured for a 5-min period”
Inter-session interval
Wait between first and second exposure sessions
View evidence from paper
“Twenty-four hours later animals were re-exposed to the same environment”
Second exposure session
Re-expose animal to the same environment and record distance walked
View evidence from paper
“Twenty-four hours later animals were re-exposed to the same environment and the distance they walked was recorded again”
Arena cleaning
Clean arena with alcohol between mice to eliminate olfactory cues
View evidence from paper
“The arenas of the Y-maze, OFH and PLT were cleaned with Alcohol between mice, in order to eliminate olfactory cues”