Source Paper
Sex-dependent effects of ultra-low-dose-THC preventive treatment on neuroinflammation and cognitive decline in 5xFAD mice
Nitzan K, Bentulila Z, Bregman-Yemini N, Ayalon N, David D et al.
Biol Sex Differ • 2026
Open-Field Habituation
behavioralMouse5xFAD and C57B/Rcc
Objective: Evaluates long-term non-associative, non-aversive spatial learning by measuring the decrease in exploratory activity
Materials & Equipment Checklist
4 items1 from ConductScience
Gather these items before starting the experiment. Check off items as you prepare.
Materials1
Not specified • Not specified
1 item available from ConductScience
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Protocol Steps
1
First exposure session
Place animal in novel open field environment and measure distance walked
5 minutes
View evidence from paper
“animals were exposed to a novel environment by placing them in a 40 cm × 40 cm × 40 cm open field box. The distance walked was measured for a 5-min period”
2
Inter-session interval
Wait between first and second exposure sessions
24 hours
View evidence from paper
“Twenty-four hours later animals were re-exposed to the same environment”
3
Second exposure session
Re-expose animal to the same environment and record distance walked
5 minutes
View evidence from paper
“Twenty-four hours later animals were re-exposed to the same environment and the distance they walked was recorded again”
4
Arena cleaning
Clean arena with alcohol between mice to eliminate olfactory cues
Not specified
View evidence from paper
“The arenas of the Y-maze, OFH and PLT were cleaned with Alcohol between mice, in order to eliminate olfactory cues”