Source Paper
Association between depression during pregnancy and preterm birth: Results from population cohorts and mouse experimental models
Chen S, Yan G, Xie X, Wang Q, Zhong J et al.
PLoS One • 2026
Open Field Test
Objective: To detect spontaneous locomotion and exploratory behavior in mice
This is a Open Field Test protocol using mouse as the model organism. The procedure involves 3 procedural steps, 1 equipment items, 1 materials. Extracted from a 2026 paper published in PLoS One.
Model and subjects
mouse • C57BL/6 • female • 6-week-old • 100
Study window
~1 hours hands-on
Core workflow
Laboratory acclimation • Individual mouse placement • Data analysis
Primary readouts
- Spontaneous locomotion
- Exploratory behavior
- Open field experiment score
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
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- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Laboratory acclimation
Allow mice to adapt to laboratory environment for 60 minutes before testing
View evidence from paper
“the mice were placed in the laboratory for 60 minutes before the start of the experiment to adapt to the laboratory environment”
Individual mouse placement
Place a single mouse in the open field test chamber for observation
View evidence from paper
“A single mouse was placed in the open field test chamber during observation”
Data analysis
Analyze open field data after completion of the overall experiment
View evidence from paper
“the open field data were analyzed after the overall experiment”