Source Paper
Unraveling sex differences in age-related hippocampal decline: differential mitochondrial dysfunction, Lonp1-dependent mitochondrial proteostasis and mtROS production in aged C57BL/6 mice
Cicali KA, Jara C, Cortés-Díaz D, Lira M, Fuentes Í et al.
Cell Death Dis • 2025
Open Field Test
behavioralMouseC57BL/6J and SAMP8
Objective: To assess locomotor activity and anxiety-like behavior by measuring distance traveled, speed, and center exploration in an open field arena
Protocol Steps
1
Test timing
Perform test during light phase of light/dark cycle
View evidence from paper
“All behavioral tests were performed in the 12 h light phase”
2
Animal placement
Place animals individually at the center of the test box
View evidence from paper
“The animals were individually placed at the center of a 72 × 72 x 32 cm white acrylic box”
3
Free movement period
Allow animals to move freely for 10 minutes
View evidence from paper
“were allowed to move freely for 10 min”
4
Data recording
Record distance traveled, speed, and center entrances during the test
View evidence from paper
“The distance traveled, speed, and number of entrances to the center of the box were recorded”