Source Paper
Unraveling sex differences in age-related hippocampal decline: differential mitochondrial dysfunction, Lonp1-dependent mitochondrial proteostasis and mtROS production in aged C57BL/6 mice
Cicali KA, Jara C, Cortés-Díaz D, Lira M, Fuentes Í et al.
Cell Death Dis • 2025
Open Field Test
Objective: To assess locomotor activity and anxiety-like behavior by measuring distance traveled, speed, and center exploration in an open field arena
This is a Open Field Test protocol using Mouse as the model organism. The procedure involves 4 procedural steps, 1 equipment items, 1 materials. Extracted from a 2025 paper published in Cell Death Dis.
Model and subjects
Mouse • C57BL/6J and SAMP8 • Male and female • 3 and 20-month-old C57BL/6J mice, and 2 and 10-month-old SAMP8 mice • 40
Study window
~10 minutes hands-on
Core workflow
Test timing • Animal placement • Free movement period
Primary readouts
- Distance traveled
- Speed
- Number of entrances to the center of the box
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
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- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Test timing
Perform test during light phase of light/dark cycle
View evidence from paper
“All behavioral tests were performed in the 12 h light phase”
Animal placement
Place animals individually at the center of the test box
View evidence from paper
“The animals were individually placed at the center of a 72 × 72 x 32 cm white acrylic box”
Free movement period
Allow animals to move freely for 10 minutes
View evidence from paper
“were allowed to move freely for 10 min”
Data recording
Record distance traveled, speed, and center entrances during the test
View evidence from paper
“The distance traveled, speed, and number of entrances to the center of the box were recorded”