Open Field Test
Objective: Assessment of spontaneous locomotor activity and exploratory behavior in light and dark phases across multiple time points in zQ175 knock-in mice
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Protocol Steps
Animal identification and genotyping
Mice were ear notched at 10-15 days of age for identification purposes
View evidence from paper
“Animals were ear notched at around 10–15 days”
RFID chip implantation
Mice were implanted with RFID electronic chips for tracking purposes
View evidence from paper
“Mice were weaned and implanted with RFID electronic chips (DataMars, OH) for identification at around 21 days”
CAG repeat genotyping
Genotyping and CAG repeat count were determined by Laragen Inc. from PCR of tail snips
View evidence from paper
“Genotyping and CAG repeat count were determined by Laragen Inc. (Culver City, CA), from PCR of tail snips taken at 10–15 days of age”
Open field test - light phase
Assessment of spontaneous locomotor activity and exploratory behavior during light phase
View evidence from paper
“Open field in the light phase ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓”
Open field test - dark phase
Assessment of spontaneous locomotor activity and exploratory behavior during dark phase
View evidence from paper
“Open field in the dark phase ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓”