Open Field Test
Objective: Measurement of exploratory behavior and locomotor activity in a novel open environment
This is a Open Field Test protocol using mouse as the model organism. The procedure involves 3 procedural steps, 1 equipment items, 4 materials. Extracted from a 2003 paper published in Journal of Neuroscience.
Model and subjects
mouse • BALB/c and C57BL/6 • not specified • adult • not specified • not specified
Study window
Estimated timing pending
Core workflow
Open field test • Novel object test • Acute drug administration
Primary readouts
- Exploratory behavior in open field
- Locomotor activity
- Behavioral responses to antipsychotic drugs (clozapine, chlorpromazine)
- Behavioral responses to psychomimetic drug (ketamine)
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
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Protocol Steps
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Open field test
Place mice in a novel open field environment to measure exploratory behavior and locomotor activity
Note: Test measures deficits in exploratory behavior compared to control mice
View evidence from paper
“deficient in exploratory behavior in both open-field and novel-object tests”
Novel object test
Place mice in environment with novel objects to measure exploratory behavior
Note: Complementary test to open field for measuring exploratory deficits
View evidence from paper
“deficient in exploratory behavior in both open-field and novel-object tests”
Acute drug administration
Administer antipsychotic drugs (clozapine, chlorpromazine) and psychomimetic drug (ketamine) acutely and observe behavioral responses
Note: Infected mice display striking responses compared to control mice
View evidence from paper
“striking responses to the acute administration of antipsychotic (clozapine and chlorpromazine) and psychomimetic (ketamine) drugs”