Source Paper
Synaptic changes contribute to persistent extra-motor behaviour deficits in amyotrophic lateral sclerosis
Luan W, San Gil R, Madrid San Martin L, Cao MC, Vassallu F et al.
Acta Neuropathol Commun • 2025
Open Field Test
Objective: Assessment of general exploratory locomotion in a novel arena
This is a Open Field Test protocol using Mouse as the model organism. The procedure involves 4 procedural steps, 1 equipment items, 2 materials. Extracted from a 2025 paper published in Acta Neuropathol Commun.
Model and subjects
Mouse • rNLS8 mice (C57BL/6JAusb background) • male and female • approximately ten weeks of age • not specified
Study window
Estimated timing pending
Core workflow
Place mouse in arena • Set illumination • Record activity for 30 minutes
Primary readouts
- General exploratory locomotion
- Time spent in different zones (periphery vs centre)
- Activity patterns analyzed in 5-minute bins
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Place mouse in arena
Place the mouse in the clear Plexiglas arena (30 cm × 30 cm × 50 cm) with white floor divided into periphery and centre zones
View evidence from paper
“novel clear Plexiglas (30 cm × 30 cm × 50 cm) arena with white floor divided by two zones”
Set illumination
Maintain illumination at 30 LUX during the test
View evidence from paper
“30 min with illumination 30 LUX”
Record activity for 30 minutes
Assess activities during a 30-minute period
View evidence from paper
“The activities were assessed during 30 min”
Analyze data in time bins
Analyze the recorded data by time using 5-minute bins
View evidence from paper
“analysed by time (bin = 5 min)”