Source Paper
Synaptic changes contribute to persistent extra-motor behaviour deficits in amyotrophic lateral sclerosis
Luan W, San Gil R, Madrid San Martin L, Cao MC, Vassallu F et al.
Acta Neuropathol Commun • 2025
Open Field Test
behavioralMouserNLS8 mice (C57BL/6JAusb background)
Objective: Assessment of general exploratory locomotion in a novel arena
Protocol Steps
1
Place mouse in arena
Place the mouse in the clear Plexiglas arena (30 cm × 30 cm × 50 cm) with white floor divided into periphery and centre zones
View evidence from paper
“novel clear Plexiglas (30 cm × 30 cm × 50 cm) arena with white floor divided by two zones”
2
Set illumination
Maintain illumination at 30 LUX during the test
View evidence from paper
“30 min with illumination 30 LUX”
3
Record activity for 30 minutes
Assess activities during a 30-minute period
View evidence from paper
“The activities were assessed during 30 min”
4
Analyze data in time bins
Analyze the recorded data by time using 5-minute bins
View evidence from paper
“analysed by time (bin = 5 min)”