Source Paper
Elucidating the Mechanism of the Liqi Yangyin Formula in Treating Depression–Constipation Comorbidity: An Integrative Approach Using Network Pharmacology and Experimental Validation
Xu L, Ong SS, Deng X, Qian Y, Tang Z et al.
Pharmaceuticals (Basel) • 2026
Open Field Test (OFT)
behavioralmouseC57BL/6
Objective: To evaluate anxiety- and locomotion-related behaviors in mice
Materials & Equipment Checklist
3 items
Protocol Steps
1
Acclimation period
Place mice in the arena for initial acclimation
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“Mice were placed in a 45 cm × 45 cm × 45 cm arena for a 5 min acclimation”
2
Return to starting position
Return mice to the starting position after acclimation
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“and then returned to the starting position for a 5 min recording session”
3
Recording session
Record mouse behavior and movement for 5 minutes using automated tracking
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“returned to the starting position for a 5 min recording session. Movements were tracked with an automated system”
4
Clean apparatus
Clean the arena with ethanol between trials
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“The apparatus was cleaned with 75% ethanol between trials to eliminate olfactory cues”