Open Field Test
Objective: Measure locomotor activity and anxiety-like behavior in an open arena as part of a chronic social defeat stress depression model to examine antidepressant effects
Gather these items before starting the experiment. Check off items as you prepare.
Equipment1
Not specified • Not specified • Not specified • Not specified
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Protocol Steps
Chronic Social Defeat Stress (CSDS) Model Implementation
Establish depression model in mice prior to behavioral testing
Note: This is the baseline condition for all subsequent behavioral tests
View evidence from paper
“A chronic social defeat stress (CSDS) depression model was performed to explore whether EDA could produce antidepressant effects”
Inhibitor Administration
Inject EX527 (Sirt1 inhibitor) and ML385 (Nrf2 inhibitor) intraperitoneally 30 minutes before EDA injection
Note: Administered daily
View evidence from paper
“EX527, a Sirt1 inhibitor and ML385, a Nrf2 inhibitor were injected intraperitoneally 30 min before EDA injection daily”
Open Field Test (OFT) Execution
Place mouse in open field arena to measure locomotor activity and anxiety-like behavior
Note: Part of behavioral test battery to examine depressive, anxiety-like and cognitive behaviors
View evidence from paper
“Behaviors tests were carried out to examine depressive, anxiety-like and cognitive behaviors including social interaction (SI) test, sucrose preference test (SPT), open field test (OFT)”