Open Field Test
Objective: Measure locomotor activity and anxiety-like behavior in an open arena as part of a chronic social defeat stress depression model to examine antidepressant effects
This is a Open Field Test protocol using mouse as the model organism. The procedure involves 3 procedural steps, 1 equipment items, 4 materials. Extracted from a 2022 paper published in Journal of Neuroinflammation.
Model and subjects
mouse • Not specified • unknown • Not specified • Not specified
Study window
~1 hours hands-on
Core workflow
Chronic Social Defeat Stress (CSDS) Model Implementation • Inhibitor Administration • Open Field Test (OFT) Execution
Primary readouts
- Locomotor activity in open arena
- Anxiety-like behavior
- Depressive behavior (measured across multiple tests)
- Cognitive behavior (measured across multiple tests)
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
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- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Chronic Social Defeat Stress (CSDS) Model Implementation
Establish depression model in mice prior to behavioral testing
Note: This is the baseline condition for all subsequent behavioral tests
View evidence from paper
“A chronic social defeat stress (CSDS) depression model was performed to explore whether EDA could produce antidepressant effects”
Inhibitor Administration
Inject EX527 (Sirt1 inhibitor) and ML385 (Nrf2 inhibitor) intraperitoneally 30 minutes before EDA injection
Note: Administered daily
View evidence from paper
“EX527, a Sirt1 inhibitor and ML385, a Nrf2 inhibitor were injected intraperitoneally 30 min before EDA injection daily”
Open Field Test (OFT) Execution
Place mouse in open field arena to measure locomotor activity and anxiety-like behavior
Note: Part of behavioral test battery to examine depressive, anxiety-like and cognitive behaviors
View evidence from paper
“Behaviors tests were carried out to examine depressive, anxiety-like and cognitive behaviors including social interaction (SI) test, sucrose preference test (SPT), open field test (OFT)”