Source Paper
Atrophin-1 antisense oligonucleotide provides robust protection from pathology in a fully humanized DRPLA model
Smith VL, Gidi BZ, Bragg RM, Cantle JP, Ben-Varon A et al.
Mol Ther Nucleic Acids • 2025
Open Field Test
behavioralMouseAtn1 Q112/+ mice
Objective: To measure locomotor activity and exploratory behavior in mice by recording distance traveled and freezing behavior in an open field arena
Protocol Steps
1
Place mouse in arena center
Place the mouse in the center of the test arena
View evidence from paper
“Mice were placed in the center of a 41 × 41 cm closed box”
2
Record video session
Record the mouse's behavior for 12 minutes using a mounted camera
View evidence from paper
“A GoPro camera, mounted in the lid, recorded a 12-min video of each individual mouse”
3
Analyze video data
Use automated software to quantify movement parameters from recorded video
View evidence from paper
“Quantitative analysis was performed using Cai Lab's ezTrack script to measure total distance traveled and the freezing to motion ratio”