Source Paper
Serotonin receptor 1A knockout: An animal model of anxiety-related disorder
Sylvie Ramboz, Ronald Oosting, Djamel Aït Amara, Hank F. Kung, Pierre Blier et al.
Proceedings of the National Academy of Sciences • 1998
Open Field Test
Objective: Assessment of exploratory activity and fear response in open or elevated spaces
This is a Open Field Test protocol using mouse as the model organism. The procedure involves 2 procedural steps, 2 equipment items. Extracted from a 1998 paper published in Proceedings of the National Academy of Sciences.
Model and subjects
mouse • 5-HT1A knockout mice and wild-type controls • Not specified • Not specified • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Place animal in open field or elevated space • Observe and record behavioral responses
Primary readouts
- Exploratory activity in open spaces
- Fear response in open or elevated spaces
- Behavioral differences between knockout, heterozygote, and wild-type mice
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Place animal in open field or elevated space
Introduce the mouse to the open field or elevated space apparatus to assess exploratory behavior and fear response
Note: The test measures both exploratory activity (decreased in knockout mice) and fear-related behaviors (increased in knockout mice)
View evidence from paper
“mice without 5-HT1A receptors display decreased exploratory activity and increased fear of aversive environments (open or elevated spaces)”
Observe and record behavioral responses
Monitor and record exploratory activity and fear-related behaviors during exposure to open or elevated spaces
Note: Heterozygote 5-HT1A mutants displayed intermediate phenotypes in behavioral tests
View evidence from paper
“Heterozygote 5-HT1A mutants expressed approximately one-half of wild-type receptor density and displayed intermediate phenotypes in most behavioral tests”