Open Field Test
Objective: Assessment of exploratory behavior and anxiety-like behavior in mice using an open field arena
This is a Open Field Test protocol using mouse as the model organism. The procedure involves 4 procedural steps, 1 equipment items. Extracted from a 2016 paper published in Nature.
Model and subjects
mouse • C57BL/6J • male • Adult (at least 6 weeks after treatment; initial treatment at 2-4.5 months) • Not specified
Study window
~2 hours hands-on
Core workflow
Animal placement in open field apparatus • Measure exploratory behavior • Measure anxiety-like behaviors
Primary readouts
- Total distance traveled (exploratory behavior/locomotion)
- Time spent rearing (anxiety-like behavior)
- Frequency of rearing (anxiety-like behavior)
Key equipment and reagents
Verified items
0
Direct vendor links
0
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Protocol Steps
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Animal placement in open field apparatus
Place mouse in the Omnitech Digiscan apparatus for spontaneous locomotion assessment
Note: The first 30 minutes of data were evaluated for all parameters
View evidence from paper
“The first 30 minutes were evaluated for all parameters”
Measure exploratory behavior
Record total distance traveled by the mouse during the 30-minute open field test session
Note: Locomotion was evaluated by the total distance traveled
View evidence from paper
“Locomotion was evaluated by the total distance traveled”
Measure anxiety-like behaviors
Assess anxiety-like behaviors by recording time spent rearing and frequency of rearing during the 30-minute session
Note: Anxiety-like behaviors were assessed by the following parameters: time spent rearing and frequency of rearing
View evidence from paper
“Anxiety-like behaviors were assessed by the following parameters: time spent rearing and frequency of rearing”
Data analysis
Perform statistical analysis on collected behavioral parameters
Note: One-way ANOVA with Bonferroni multiple comparison tests was used for statistical analysis
View evidence from paper
“Statistical analysis was done using one-way ANOVA with Bonferroni multiple comparison tests”