Source Paper
Electroacupuncture promotes BDNF-dependent neurogenesis via microglial reprogramming in a chronic stress model
Zhang L, Wei T, Liu X, Zhang L, Wang D et al.
Chin Med • 2026
Open Field Test
behavioralMouseC57BL/6J
Objective: Assess general locomotor activity and anxiety-like behavior
Protocol Steps
1
Pre-test handling and acclimation
Handle mice for three consecutive days to reduce handling stress. Transfer mice to testing room 1 hour in advance for acclimation on test day.
1 hour acclimation
View evidence from paper
“all mice were handled for three consecutive days to reduce handling stress”
2
Place mouse in arena
Place mouse in the white, rectangular open-field arena for behavioral recording.
Not specified
View evidence from paper
“white, rectangular open-field arena (50 × 50 × 50 cm)”
3
Record locomotor activity
Record mouse locomotor activity using video tracking software for analysis of movement patterns and anxiety-like behaviors.
5 min
View evidence from paper
“Locomotor activity was recorded for 5 min in a white, rectangular open-field arena”