Source Paper
Electroacupuncture promotes BDNF-dependent neurogenesis via microglial reprogramming in a chronic stress model
Zhang L, Wei T, Liu X, Zhang L, Wang D et al.
Chin Med • 2026
Open Field Test
Objective: Assess general locomotor activity and anxiety-like behavior
This is a Open Field Test protocol using Mouse as the model organism. The procedure involves 3 procedural steps, 2 equipment items. Extracted from a 2026 paper published in Chin Med.
Model and subjects
Mouse • C57BL/6J • Male • 8-week-old • Variable by experiment group
Study window
~2.1 hours hands-on
Core workflow
Pre-test handling and acclimation • Place mouse in arena • Record locomotor activity
Primary readouts
- Total distance traveled
- Number of zone transitions
- Time spent in the central zone
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
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- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
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Pre-test handling and acclimation
Handle mice for three consecutive days to reduce handling stress. Transfer mice to testing room 1 hour in advance for acclimation on test day.
View evidence from paper
“all mice were handled for three consecutive days to reduce handling stress”
Place mouse in arena
Place mouse in the white, rectangular open-field arena for behavioral recording.
View evidence from paper
“white, rectangular open-field arena (50 × 50 × 50 cm)”
Record locomotor activity
Record mouse locomotor activity using video tracking software for analysis of movement patterns and anxiety-like behaviors.
View evidence from paper
“Locomotor activity was recorded for 5 min in a white, rectangular open-field arena”