Source Paper
Aerobic Exercise Promotes Hippocampal Neurogenesis and Ameliorates Cognitive Dysfunction Induced by Unilateral Labyrinthectomy
Zhou Z, Yu X, Tian E, Guo Z, Chen J et al.
CNS Neurosci Ther • 2026
Open-Field Test
Objective: To assess general locomotor activity and exploratory behavior in mice by measuring total distance traveled in an open arena
This is a Open-Field Test protocol using Mouse as the model organism. The procedure involves 4 procedural steps, 2 equipment items, 1 materials. Extracted from a 2026 paper published in CNS Neurosci Ther.
Model and subjects
Mouse • C57BL/6J • Male • 8 weeks old • Not specified
Study window
~3 day study window | ~15 minutes hands-on
Core workflow
Pre-test acclimation • Mouse placement • Movement monitoring
Primary readouts
- Total distance traveled during the 5-minute session
Key equipment and reagents
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Protocol Steps
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Pre-test acclimation
Handle mice for a minimum of 5 minutes, twice daily, for three consecutive days prior to testing
View evidence from paper
“mice were acclimated by handling for a minimum of 5 min, twice daily, for three consecutive days”
Mouse placement
Place mouse at the center of the rectangular chamber
View evidence from paper
“Mice were placed at the center of a rectangular chamber”
Movement monitoring
Monitor and record mouse movement using automated video tracking system
View evidence from paper
“their movement was monitored for 5 min using an automated video tracking system”
Apparatus cleaning
Clean the apparatus with 75% alcohol between trials to mitigate olfactory cues
View evidence from paper
“the apparatus was cleaned with 75% alcohol following each trial”