Source Paper
Aerobic Exercise Promotes Hippocampal Neurogenesis and Ameliorates Cognitive Dysfunction Induced by Unilateral Labyrinthectomy
Zhou Z, Yu X, Tian E, Guo Z, Chen J et al.
CNS Neurosci Ther • 2026
Open-Field Test
behavioralMouseC57BL/6J
Objective: To assess general locomotor activity and exploratory behavior in mice by measuring total distance traveled in an open arena
Materials & Equipment Checklist
3 items
Gather these items before starting the experiment. Check off items as you prepare.
Materials1
Not specified • Not specified
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Protocol Steps
1
Pre-test acclimation
Handle mice for a minimum of 5 minutes, twice daily, for three consecutive days prior to testing
5 minutes per session, twice daily for 3 days
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“mice were acclimated by handling for a minimum of 5 min, twice daily, for three consecutive days”
2
Mouse placement
Place mouse at the center of the rectangular chamber
Not specified
View evidence from paper
“Mice were placed at the center of a rectangular chamber”
3
Movement monitoring
Monitor and record mouse movement using automated video tracking system
5 minutes
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“their movement was monitored for 5 min using an automated video tracking system”
4
Apparatus cleaning
Clean the apparatus with 75% alcohol between trials to mitigate olfactory cues
Not specified
View evidence from paper
“the apparatus was cleaned with 75% alcohol following each trial”