Source Paper
Lack of striatal-enriched protein tyrosine phosphatase affected the serotonin system, behavior, and brain morphology in mice
Moskaliuk V, Komleva P, Khotskin N, Arefieva A, Shevelev O et al.
Front Psychiatry • 2026
Operant Wall
Objective: To evaluate associative learning capabilities in mice using an operant conditioning paradigm with food pellet rewards
This is a Operant Wall protocol using Mouse as the model organism. The procedure involves 4 procedural steps, 1 equipment items, 1 materials. Extracted from a 2026 paper published in Front Psychiatry.
Model and subjects
Mouse • C57BL/6-Ptpn5_KO and C57BL/6 • Male • 2-month-old • Not specified
Study window
Estimated timing pending
Core workflow
Setup timing • Habituation day • Task training
Primary readouts
- Total number of received pellets
- Total number of performed nose pokes
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
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- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
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Setup timing
Activate the operant wall during specific hours while mice are in the PhenoMaster-equipped cage
View evidence from paper
“The operant wall was turned on from 15:30 to 17:30 during the mice's presence in the PhenoMaster-equipped cage”
Habituation day
Provide free pellet reward without requiring any task performance to familiarize animals with the reward
View evidence from paper
“As animals were not subjected to food deprivation, to arouse their interest and familiarize with the reward, a pellet was dispensed without any tasks at habituation day”
Task training
Require animals to perform tasks to obtain food pellet rewards over 3 consecutive days
View evidence from paper
“During the next 3 days, to get the reward, the animal had to perform tasks”
Data collection
Record the total number of pellets received and nose pokes performed during days 2 and 3 of task training
View evidence from paper
“To assess the learning capabilities, the total number of received pellets and performed nose pokes were recorded during the task of days 2 and 3”