Source Paper
Short-Chain Fatty Acids Stimulate Glucagon-Like Peptide-1 Secretion via the G-Protein–Coupled Receptor FFAR2
Gwen Tolhurst, Helen Heffron, Yu Shan Lam, Helen E. Parker, Abdella M. Habib et al.
Diabetes • 2011
Oral Glucose Tolerance Test
Objective: To assess glucose tolerance in ffar2 and ffar3 knockout mice compared to wild-type controls by measuring blood glucose and insulin levels following oral glucose administration
Gather these items before starting the experiment. Check off items as you prepare.
Equipment1
OneTouch Ultra
Software2
GraphPad
Microsoft
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Protocol Steps
Animal fasting
Three- to four-month-old ffar2, ffar3, and wild-type littermate control 129/SvEv mice were fasted
Note: Fasting period prior to glucose administration
View evidence from paper
“Three- to four-month-old ffar2 −/−, ffar3 −/−, and wild-type littermate control 129/SvEv mice were fasted for 14 h”
Oral glucose administration
Mice were dosed by gavage with glucose solution
Note: Administered via oral gavage after fasting period
View evidence from paper
“then dosed by gavage with 1.5 g/kg glucose”
Blood glucose measurement
Blood glucose was measured using a handheld glucometer via tail bleed from awake mice
Note: Measurements taken at multiple time points following glucose dosing
View evidence from paper
“Blood glucose was measured using a handheld glucometer (OneTouch Ultra) via tail bleed”
Insulin sample collection
Blood samples were collected via tail bleed from awake mice into heparinized capillary tubes
Note: Samples collected for insulin measurements at specified time points
View evidence from paper
“Samples for insulin measurements were taken via tail bleed from awake mice into heparinized capillary tubes (Bilbate)”
Insulin assay
Insulin levels were measured using ELISA
View evidence from paper
“assayed by ELISA (Crystalchem Ultra sensitive mouse insulin ELISA)”