Pair Feeding Study
Objective: To determine whether ghrelin-induced adiposity is due to increased energy intake by comparing body weight and fat mass changes in pair-fed versus ad libitum-fed mice receiving ghrelin administration
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Equipment1
Not specified • Not specified • Not specified • Not mentioned
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Protocol Steps
Ghrelin administration under high fat diet
Repeated administrations of ghrelin were given to mice maintained on a high fat diet
Note: This was the primary intervention to induce adiposity
View evidence from paper
“Body weight, fat mass, glucose, insulin measured after repeated administrations of ghrelin under a high fat diet”
Pair feeding intervention
Pair feeding was implemented to determine if ghrelin-induced adiposity effects could be inhibited by controlling energy intake
Note: Pair feeding inhibited the adiposity effect of ghrelin
View evidence from paper
“Pair feeding inhibited this effect”
Measurement of body weight and fat mass
Body weight and fat mass were measured following ghrelin administration
Note: Measurements were taken after repeated ghrelin administrations
View evidence from paper
“Body weight, fat mass, glucose, insulin measured after repeated administrations of ghrelin under a high fat diet”
Measurement of glucose and insulin
Glucose and insulin levels were measured to assess glycaemic control
Note: Ghrelin worsened glycaemic control under high fat diet
View evidence from paper
“Body weight, fat mass, glucose, insulin measured after repeated administrations of ghrelin under a high fat diet”
Gene expression analysis in white adipose tissue
mRNA expression of leptin, adiponectin, and resistin in white adipose tissue was measured
Note: Ghrelin elevated leptin mRNA and reduced resistin mRNA expression
View evidence from paper
“gene expression of leptin, adiponectin, and resistin in white adipose tissue were measured”
Gastric ghrelin gene expression assessment
Gastric ghrelin mRNA expression during fasting was assessed by northern blot analysis
Note: High fat diet increased gastric ghrelin mRNA expression during fasting
View evidence from paper
“Gastric ghrelin gene expression was assessed by northern blot analysis”
Energy intake measurement after GHS-R antagonist administration
Energy intake was measured in lean mice, diet-induced obese mice, and ob/ob obese mice following GHS-R antagonist administration
Note: GHS-R antagonists decreased energy intake across all mouse types tested
View evidence from paper
“Energy intake measured after administration of GHS-R antagonists”
Gastric emptying measurement
Rate of gastric emptying was measured following GHS-R antagonist administration
Note: GHS-R antagonists reduced the rate of gastric emptying
View evidence from paper
“gastric emptying were measured after administration of GHS-R antagonists”
Repeated GHS-R antagonist administration in ob/ob mice
GHS-R antagonist was repeatedly administered to ob/ob obese mice
Note: This treatment decreased body weight gain and improved glycaemic control
View evidence from paper
“Repeated administration of GHS-R antagonist was continued for six days in ob/ob obese mice”