Source Paper
The Nuclear Kinase Mitogen- and Stress-Activated Protein Kinase 1 Regulates Hippocampal Chromatin Remodeling in Memory Formation
Wilson B. Chwang, J. Simon Arthur, Armin Schumacher, J. David Sweatt
Journal of Neuroscience • 2007
Pavlovian Fear Conditioning
Objective: Assessment of fear memory formation and retention in mice using Pavlovian fear conditioning paradigm to investigate the role of MSK1 in chromatin remodeling during hippocampus-dependent memory formation
This is a Pavlovian Fear Conditioning protocol using mouse as the model organism. The procedure involves 6 procedural steps, 2 equipment items, 1 materials. Extracted from a 2007 paper published in Journal of Neuroscience.
Model and subjects
mouse • Not explicitly stated • unknown • Not explicitly stated • Not explicitly stated
Study window
Estimated timing pending
Core workflow
Pavlovian Fear Conditioning Training • Hippocampal Tissue Collection • Hippocampal Slice Preparation
Primary readouts
- Pavlovian fear conditioning performance
- Spatial learning ability
- Histone phosphorylation levels in hippocampus
- Histone acetylation levels in hippocampus
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
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- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
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Pavlovian Fear Conditioning Training
Mice undergo fear conditioning training to assess memory formation
Note: Both MSK1 knock-out and wild-type control mice are tested
View evidence from paper
“Mice lacking MSK1 show impaired Pavlovian fear conditioning and spatial learning”
Hippocampal Tissue Collection
Hippocampal tissue is collected after fear training for analysis of histone phosphorylation and acetylation
Note: Tissue analyzed for histone modifications and CREB phosphorylation
View evidence from paper
“a deficiency in histone phosphorylation and acetylation in the hippocampus after fear training”
Hippocampal Slice Preparation
Hippocampal slices are prepared from MSK1 knock-out mice for in vitro studies
Note: Slices are used to test ERK pathway activation effects
View evidence from paper
“hippocampal slices from MSK1 knock-out mice exhibit a deficiency in both histone phosphorylation and acetylation after activation of the ERK pathway in vitro”
ERK Pathway Activation
ERK pathway is activated in hippocampal slices to assess downstream effects on histone modifications
Note: In vitro activation study comparing MSK1 knock-out to wild-type
View evidence from paper
“hippocampal slices from MSK1 knock-out mice exhibit a deficiency in both histone phosphorylation and acetylation after activation of the ERK pathway in vitro”
Sodium Butyrate Injection
In vivo injections of sodium butyrate (histone deacetylase inhibitor) are administered to MSK1 knock-out mice
Note: Treatment does not alleviate fear conditioning deficit, suggesting CREB phosphorylation is critical independent of histone acetylation
View evidence from paper
“In vivo injections of a histone deacetylase inhibitor, sodium butyrate, fail to alleviate the fear conditioning deficit in MSK1 knock-out mice”
Biochemical Analysis
Analysis of histone phosphorylation, histone acetylation, and CREB phosphorylation in hippocampal tissue
Note: CREB phosphorylation deficiency persists after sodium butyrate injection
View evidence from paper
“MSK1 knock-out mice demonstrate a deficiency in cAMP response element-binding protein (CREB) phosphorylation after fear training, which persists after sodium butyrate injection”