Peripheral Leptin Administration and Food Intake Measurement — M Van Heek | ReplicateScience

ReplicateScience

Submit Paper ConductScience

Peripheral Leptin Administration and Food Intake Measurement — M Van Heek | ReplicateScience

1

Induce obesity through high fat diet exposure

Expose two strains of mice (C57BL/6 and AKR) to a 45% fat diet to induce obesity

Not specifiedNot specified

Note: Two dietary conditions were used: 45% fat diet and 10% fat diet control

View evidence from paper

“Obesity was induced in two strains of mice by exposure to a 45% fat diet”

2

Measure baseline serum leptin levels

Collect serum and measure leptin levels in relation to body weight

Not specifiedNot specified

Note: Serum leptin increased in proportion to body weight with statistical significance

View evidence from paper

“Serum leptin increased in proportion to body weight (P < 0.00001)”

3

Administer peripheral leptin to C57BL/6 mice

Administer leptin peripherally to C57BL/6 mice on high fat diet and measure food intake response

16 days of dietary treatmentNot specified

Note: Initial response observed but leptin resistance developed after 16 days on high fat diet

View evidence from paper

“Whereas C57BL/6 mice initially responded to peripherally administered leptin with a marked decrease in food intake, leptin resistance developed after 16 d on high fat diet”

4

Administer peripheral leptin to C57BL/6 mice on low fat diet

Administer leptin peripherally to C57BL/6 mice on 10% fat diet as control

Not specifiedNot specified

Note: Mice on low fat diet retained leptin sensitivity

View evidence from paper

“mice on 10% fat diet retained leptin sensitivity”

5

Administer peripheral leptin to AKR mice

Administer leptin peripherally to AKR mice on both 10% and 45% fat diets and measure food intake response

16 days and 56 days of dietary treatmentNot specified

Note: AKR mice showed initial response at 16 days but developed resistance by 56 days on both diets

View evidence from paper

“In AKR mice, peripheral leptin significantly decreased food intake in both 10 and 45% fat-fed mice after 16 d of dietary treatment. However, after 56 d, both groups became resistant to peripherally administered leptin”

6

Administer central leptin via intracerebroventricular infusion

Administer leptin centrally via single intracerebroventricular infusion to peripherally leptin-resistant AKR mice on 45% fat diet

Single infusionNot specified

Note: Central leptin administration produced dose-dependent responses in food intake and body weight

View evidence from paper

“Central administration of leptin to peripherally leptin-resistant AKR mice on 45% fat diet resulted in a robust response to leptin, with a dose-dependent decrease in food intake (P < 0.00001) and body weight (P < 0.0001) after a single intracerebroventricular infusion”

7

Measure food intake and body weight changes

Monitor and record food intake and body weight changes in response to leptin administration

Throughout experimental periodNot specified

Note: Food intake and body weight were primary outcome measures

View evidence from paper

“with a dose-dependent decrease in food intake (P < 0.00001) and body weight (P < 0.0001)”