Permanent Distal Middle Cerebral Artery Occlusion with Hypoxia
Objective: Induce ischemic stroke in mice through permanent distal middle cerebral artery occlusion combined with hypoxia, and evaluate neurological deficits, angiogenesis, neural plasticity, microglial polarization, and inflammatory responses
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Materials1
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Protocol Steps
Ischemic stroke induction
Induce ischemic stroke in mice using permanent distal middle cerebral artery occlusion combined with hypoxia exposure
Note: Procedure involves permanent distal MCAO with concurrent hypoxia
View evidence from paper
“Ischemic stroke mice model was induced by permanent distal middle cerebral artery occlusion plus 1 h hypoxia”
TWS119 administration
Administer TWS119 to stroke-induced mice
Note: Treatment period spans 14 days following stroke induction
View evidence from paper
“TWS119 was administrated from day 1 to 14 after stroke”
Neurological deficit monitoring
Monitor and assess neurological deficits in stroke-induced mice
Note: Continuous monitoring period extends beyond treatment period
View evidence from paper
“Neurological deficits were monitored up to 21 days after stroke”
Tissue analysis at day 14
Collect and analyze peri-infarct cortex tissue for angiogenesis, neural plasticity, microglial polarization, and microglia-associated inflammatory cytokines
Note: First timepoint for molecular analysis
View evidence from paper
“Angiogenesis, neural plasticity, microglial polarization, and microglia-associated inflammatory cytokines were detected in the peri-infarct cortex at days 14 and 21 after stroke”
Tissue analysis at day 21
Collect and analyze peri-infarct cortex tissue for angiogenesis, neural plasticity, microglial polarization, and microglia-associated inflammatory cytokines
Note: Second timepoint for molecular analysis
View evidence from paper
“Angiogenesis, neural plasticity, microglial polarization, and microglia-associated inflammatory cytokines were detected in the peri-infarct cortex at days 14 and 21 after stroke”
In vitro mechanistic studies with primary microglia
Employ primary microglia to explore underlying mechanisms
Note: Cell culture-based investigation
View evidence from paper
“Primary microglia and mouse brain microvascular endothelial cell lines were employed to explore the underlying mechanism in vitro”
In vitro mechanistic studies with endothelial cells
Employ mouse brain microvascular endothelial cell lines to explore underlying mechanisms
Note: Cell culture-based investigation
View evidence from paper
“Primary microglia and mouse brain microvascular endothelial cell lines were employed to explore the underlying mechanism in vitro”