PhenoCube Assessment
Objective: Comprehensive automated behavioral phenotyping assessment of zQ175 Huntington's disease model mice at specific developmental timepoints using the PhenoCube system
Protocol Steps
Animal identification and genotyping
Mice were ear notched at 10-15 days of age for identification purposes
View evidence from paper
“Animals were ear notched at around 10–15 days”
RFID chip implantation
Mice were implanted with RFID electronic chips for tracking purposes
View evidence from paper
“Mice were weaned and implanted with RFID electronic chips (DataMars, OH) for identification at around 21 days”
Genotyping and CAG repeat determination
PCR analysis of tail snips was performed to determine genotype and CAG repeat count
View evidence from paper
“Genotyping and CAG repeat count were determined by Laragen Inc. (Culver City, CA), from PCR of tail snips taken at 10–15 days of age”
PhenoCube behavioral assessment - Cohort 2
Secondary cohort (7-16 animals per sex per genotype) underwent PhenoCube testing at unspecified age
View evidence from paper
“two secondary groups of animals (each: 7–16/sex/genotype) evaluated in the PhenoCube and swim tank”
PhenoCube behavioral assessment - Cohort 3
Secondary cohort (7-16 animals per sex per genotype) underwent PhenoCube testing at 37 weeks of age
View evidence from paper
“the 41 week collection was taken from animals evaluated in the PhenoCube at 37 weeks (see cohort 3 in Table 1)”
Tissue collection - timepoint 1
Tissue collected from behaviorally naive satellite groups
View evidence from paper
“Tissue was collected at three timepoints, at 12, 18 and 41 weeks of age, with an n=5–12 per group. Tissue from the 12 and 18 week timepoints was collected from behaviorally naïve satellite groups”
Tissue collection - timepoint 2
Tissue collected from behaviorally naive satellite groups
View evidence from paper
“Tissue was collected at three timepoints, at 12, 18 and 41 weeks of age, with an n=5–12 per group. Tissue from the 12 and 18 week timepoints was collected from behaviorally naïve satellite groups”
Tissue collection - timepoint 3
Tissue collected from animals previously evaluated in PhenoCube at 37 weeks
View evidence from paper
“the 41 week collection was taken from animals evaluated in the PhenoCube at 37 weeks”