Source Paper
Sex-dependent effects of ultra-low-dose-THC preventive treatment on neuroinflammation and cognitive decline in 5xFAD mice
Nitzan K, Bentulila Z, Bregman-Yemini N, Ayalon N, David D et al.
Biol Sex Differ • 2026
Place Recognition Test
Objective: Tests long-term memory and utilizes the natural tendency of mice to explore novel stimuli
This is a Place Recognition Test protocol using mouse as the model organism. The procedure involves 4 procedural steps, 2 equipment items, 3 materials. Extracted from a 2026 paper published in Biol Sex Differ.
Model and subjects
mouse • 5xFAD and C57B/Rcc • male and female • 6 months • 80
Study window
~10 minutes hands-on
Core workflow
Familiarization session • Inter-session interval • Test session
Primary readouts
- Time spent exploring each object during test session
- Place recognition performance (preference for object in new location)
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
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Protocol Steps
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Familiarization session
Place mouse in open field box with two identical objects and allow exploration
View evidence from paper
“During the familiarization session, the mouse was left to explore two identical objects for 5 min”
Inter-session interval
Wait between familiarization and test sessions
View evidence from paper
“24 h later, the mouse was introduced into the arena for the test session”
Test session
Place one familiar object in new location and record exploration time of each object
View evidence from paper
“one of the familiar objects was placed in a new location and The time spent exploring each object was recorded for 5 min”
Arena cleaning
Clean arena between mice to eliminate olfactory cues
View evidence from paper
“The arenas of the Y-maze, OFH and PLT were cleaned with Alcohol between mice”