Source Paper
Sex-dependent effects of ultra-low-dose-THC preventive treatment on neuroinflammation and cognitive decline in 5xFAD mice
Nitzan K, Bentulila Z, Bregman-Yemini N, Ayalon N, David D et al.
Biol Sex Differ • 2026
Place Recognition Test
behavioralmouse5xFAD and C57B/Rcc
Objective: Tests long-term memory and utilizes the natural tendency of mice to explore novel stimuli
Materials & Equipment Checklist
5 items1 from ConductScience
Gather these items before starting the experiment. Check off items as you prepare.
1 item available from ConductScience
As an Amazon Associate, we earn from qualifying purchases. Product links help support this free resource.
Protocol Steps
1
Familiarization session
Place mouse in open field box with two identical objects and allow exploration
5 min
View evidence from paper
“During the familiarization session, the mouse was left to explore two identical objects for 5 min”
2
Inter-session interval
Wait between familiarization and test sessions
24 h
View evidence from paper
“24 h later, the mouse was introduced into the arena for the test session”
3
Test session
Place one familiar object in new location and record exploration time of each object
5 min
View evidence from paper
“one of the familiar objects was placed in a new location and The time spent exploring each object was recorded for 5 min”
4
Arena cleaning
Clean arena between mice to eliminate olfactory cues
not specified
View evidence from paper
“The arenas of the Y-maze, OFH and PLT were cleaned with Alcohol between mice”