Source Paper
Fecal microbiota transplantation protects rotenone-induced Parkinson’s disease mice via suppressing inflammation mediated by the lipopolysaccharide-TLR4 signaling pathway through the microbiota-gut-brain axis
Zhe Zhao, Jingwen Ning, Xiu-qi Bao, Meiyu Shang, Jingwei Ma et al.
Microbiome • 2021
Pole Test
Objective: Assessment of motor function and coordination by measuring time to descend a vertical pole
This is a Pole Test protocol using mouse as the model organism. The procedure involves 5 procedural steps, 1 equipment items, 3 materials. Extracted from a 2021 paper published in Microbiome.
Model and subjects
mouse • C57BL/6J • male • 8 weeks • 20-22 g • 45
Study window
~4 week study window
Core workflow
Animal acclimation • Group assignment and treatment initiation • Secondary group division and treatment continuation
Primary readouts
- Time to descend the vertical pole
- Motor function assessment
- Motor coordination assessment
Key equipment and reagents
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Protocol Steps
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Animal acclimation
Mice were acclimated under standard conditions with 12-hour light/dark cycle, temperature 22±2°C, humidity 50-60%, with ad libitum access to food and water
Note: Humidity maintained at 50-60%
View evidence from paper
“The mice were then acclimatized (12-h light/dark cycle) under standard conditions (temperature 22 ± 2 °C, humidity 50–60%) with ad libitum access to food and water for 7 days”
Group assignment and treatment initiation
45 mice randomly assigned into control group (n=15) and model group (n=30). Model group received daily oral rotenone administration; control group received vehicle
Note: Mice were weighed daily during this period
View evidence from paper
“A total of 45 mice were randomly assigned into two groups: the control group (n = 15) and the model group (n = 30). In the beginning 4 weeks, the model group received the oral administration of rotenone every day”
Secondary group division and treatment continuation
After 4 weeks, model group mice were randomly divided into Rotenone group (n=15) and FMT group (n=15). During weeks 5-6, FMT group received FMT once daily; control and rotenone groups received vehicle once daily
Note: Mice continued to be weighed daily
View evidence from paper
“After 4 weeks, we randomly divided the model group mice into two groups: Rotenone group (n = 15) and FMT group (n = 15). During week 5 and 6, the mice in the FMT group were treated with FMT once per day”
Pole test performance
At week 6, mice were tested on the pole test to measure time to descend a vertical pole as an assessment of motor function and coordination
Note: Performed at week 6 along with other behavioral tests
View evidence from paper
“In addition, GI function tests and behavioral tests were performed at week 6”
Animal sacrifice and tissue collection
All mice were sacrificed at week 6 for further analysis
View evidence from paper
“All the mice were sacrificed at week 6 for further analysis”