Source Paper
Behavioral effects of a chronic envy-like stress paradigm in mice using an adjacent cage model
Ueno H, Tanaka Y, Kitano E, Takahashi Y, Mori S et al.
IBRO Neurosci Rep • 2026
Porsolt forced-swim test
behavioralMouseC57BL/6 N
Objective: To evaluate depressive-like behavior using the Porsolt forced swim test
Materials & Equipment Checklist
5 items
Protocol Steps
1
Setup apparatus
Fill Plexiglas cylinder with water at specified temperature and depth
View evidence from paper
“filled with water maintained at 23 °C to a depth of 7.5 cm”
2
Place mouse in cylinder
Place each mouse in the water-filled cylinder
View evidence from paper
“Each mouse was placed in the cylinder for 6 min”
3
Record behavior
Record mouse behavior for 6 minutes using video camera
View evidence from paper
“behavior was recorded with a video camera”
4
Single testing
Test each mouse only once
View evidence from paper
“Each mouse was tested once”
5
Quantify immobility
Measure immobility time using ANY-maze software
View evidence from paper
“immobility time (s) was quantified, with data acquisition and analysis performed using ANY-maze software”