Source Paper
Behavioral effects of a chronic envy-like stress paradigm in mice using an adjacent cage model
Ueno H, Tanaka Y, Kitano E, Takahashi Y, Mori S et al.
IBRO Neurosci Rep • 2026
Porsolt forced-swim test
Objective: To evaluate depressive-like behavior using the Porsolt forced swim test
This is a Porsolt forced-swim test protocol using Mouse as the model organism. The procedure involves 5 procedural steps, 3 equipment items, 2 materials. Extracted from a 2026 paper published in IBRO Neurosci Rep.
Model and subjects
Mouse • C57BL/6 N • Male • Eight-week-old • 10
Study window
~6 minutes hands-on
Core workflow
Setup apparatus • Place mouse in cylinder • Record behavior
Primary readouts
- Immobility time (seconds)
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
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Protocol Steps
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Setup apparatus
Fill Plexiglas cylinder with water at specified temperature and depth
View evidence from paper
“filled with water maintained at 23 °C to a depth of 7.5 cm”
Place mouse in cylinder
Place each mouse in the water-filled cylinder
View evidence from paper
“Each mouse was placed in the cylinder for 6 min”
Record behavior
Record mouse behavior for 6 minutes using video camera
View evidence from paper
“behavior was recorded with a video camera”
Single testing
Test each mouse only once
View evidence from paper
“Each mouse was tested once”
Quantify immobility
Measure immobility time using ANY-maze software
View evidence from paper
“immobility time (s) was quantified, with data acquisition and analysis performed using ANY-maze software”