Prepulse Inhibition of Acoustic Startle Response
Objective: Measurement of sensorimotor gating through reduced startle response to a strong stimulus preceded by a weaker prepulse
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Equipment1
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Protocol Steps
Prepulse Inhibition Test Setup
Conduct prepulse inhibition of acoustic startle response testing as part of the standardized operating procedures
Note: This test was one of seven behavioral tests included in the EUMORPHIA standard operating procedures battery
View evidence from paper
“tests designed provide reliable measures of the process under investigation but most importantly that these are reproducible across both time and laboratories”
Cross-Laboratory Validation
Conduct the prepulse inhibition test across five research centers in France, Germany, Italy, and the UK to validate reproducibility
Note: Part of EUMORPHIA program cross-validation experimental study to investigate robustness of designed protocols
View evidence from paper
“Five research centers were involved across France, Germany, Italy, and the UK in this study, as part of the EUMORPHIA program”
Test Four Inbred Reference Strains
Analyze four inbred reference strains to validate the prepulse inhibition test
Note: Strains used: C57BL/6J, C3HeB/FeJ, BALB/cByJ, 129S2/SvPas
View evidence from paper
“Four inbred reference strains (C57BL/6J, C3HeB/FeJ, BALB/cByJ, 129S2/SvPas), reflecting their use as common background strains in mutagenesis programs, were analyzed”