Procedural 2-Choice Swim Tank Test
Objective: Assessment of procedural learning and cognitive function using a two-choice swimming task
This is a Procedural 2-Choice Swim Tank Test protocol using mouse as the model organism. The procedure involves 3 procedural steps, 1 equipment items, 1 materials. Extracted from a 2012 paper published in PLoS ONE.
Model and subjects
mouse • C57BL/6J background with zQ175 CAG repeat expansion • both • Testing age not explicitly specified for swim tank test in provided text • not specified • 7-16 per sex per genotype in secondary cohort
Study window
~3 week study window
Core workflow
Animal identification and genotyping • RFID chip implantation • Procedural 2-choice swim tank test
Primary readouts
- Procedural learning performance in two-choice swimming task
- Cognitive function assessment
Key equipment and reagents
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Protocol Steps
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Animal identification and genotyping
Mice were ear notched at around 10-15 days of age for identification purposes
Note: Genotyping and CAG repeat count were determined by Laragen Inc. from PCR of tail snips taken at 10-15 days of age
View evidence from paper
“Animals were ear notched at around 10–15 days. Genotyping and CAG repeat count were determined by Laragen Inc. (Culver City, CA), from PCR of tail snips taken at 10–15 days of age.”
RFID chip implantation
Mice were implanted with RFID electronic chips for identification and tracking
Note: Performed at weaning
View evidence from paper
“Mice were weaned and implanted with RFID electronic chips (DataMars, OH) for identification at around 21 days.”
Procedural 2-choice swim tank test
Secondary cohort of animals (7-16 per sex per genotype) underwent the procedural 2-choice swim tank test
Note: This test was part of the behavioral evaluation in secondary cohorts. Specific testing age and detailed procedural steps are not provided in the extracted text.
View evidence from paper
“two secondary groups of animals (each: 7–16/sex/genotype) evaluated in the PhenoCube and swim tank”