Radial Arm Water Maze
Objective: Assessment of spatial memory performance in mice following whole brain irradiation using the radial arm water maze
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Protocol Steps
Whole Brain Irradiation Treatment
C56BL/6 mice at 3 months of age were subjected to fractionated whole brain irradiation protocol
Note: Fractionated protocol: 5 Gy twice weekly for 4 weeks. Control mice were also included for comparison.
View evidence from paper
“C56BL/6 mice (3 months) subjected to a clinically relevant protocol of fractionated WBI (5 Gy twice weekly for 4 weeks)”
Spatial Memory Testing - Radial Arm Water Maze
Mice were tested for spatial memory performance using the radial arm water maze
Note: Testing occurred at 3 to 6 months post-irradiation
View evidence from paper
“Mice were tested for spatial memory performance (radial arm water maze), sensorimotor coordination (computerized gait analysis, CatWalk), and cerebromicrovascular function at 3 to 6 months post-irradiation”
Sensorimotor Coordination Testing - CatWalk
Mice were tested for sensorimotor coordination using computerized gait analysis
Note: Testing occurred at 3 to 6 months post-irradiation. Gait parameters measured included regularity index and phase dispersion.
View evidence from paper
“sensorimotor coordination (computerized gait analysis, CatWalk), and cerebromicrovascular function (whisker-stimulation-induced increases in CBF, measured by laser Doppler flowmetry) at 3 to 6 months post-irradiation”
Cerebromicrovascular Function Assessment
Measurement of cerebral blood flow changes in response to whisker stimulation using laser Doppler flowmetry
Note: Testing occurred at 3 to 6 months post-irradiation
View evidence from paper
“cerebromicrovascular function (whisker-stimulation-induced increases in CBF, measured by laser Doppler flowmetry) at 3 to 6 months post-irradiation”