Experiments/Rearing and Climbing Assessment

Source Paper

Comprehensive Behavioral and Molecular Characterization of a New Knock-In Mouse Model of Huntington’s Disease: zQ175

Liliana B. Menalled, Andrea E. Kudwa, Sam Miller, Jon Fitzpatrick, Judy Watson-Johnson et al.

PLoS ONE • 2012

DOI PMC

Rearing and Climbing Assessment

behavioralmouseC57BL/6J background with zQ175 CAG repeat expansion

Objective: Measurement of vertical exploratory behavior and climbing activity in light and dark phases across different ages in zQ175 knock-in mice

This is a Rearing and Climbing Assessment protocol using mouse as the model organism. The procedure involves 5 procedural steps, 1 equipment items, 1 materials. Extracted from a 2012 paper published in PLoS ONE.

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Replication Essentials

Model and subjects

mouse • C57BL/6J background with zQ175 CAG repeat expansion • both male and female • 4-38 weeks (multiple timepoints: weeks 8, 9, 12, 13, 16, 17, 20, 21, 22, 24, 25, 28, 29, 30, 32, 33, 36, 37, 38) • 9-12 per sex per genotype in primary test cohort

Study window

~2.1 week study window

Core workflow

Animal identification and genotyping • RFID chip implantation • Genotyping and CAG repeat determination

Primary readouts

Rearing behavior (vertical exploratory activity)
Climbing activity
Light phase measurements
Dark phase measurements

Key equipment and reagents

PhenoCubeRFID electronic identification chips

Before You Start

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Protocol Steps

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Animal identification and genotyping

Mice were ear notched at 10-15 days of age for identification purposes

View evidence from paper

“Animals were ear notched at around 10–15 days”

RFID chip implantation

Mice were implanted with RFID electronic chips for tracking purposes

View evidence from paper

“Mice were weaned and implanted with RFID electronic chips (DataMars, OH) for identification at around 21 days”

Genotyping and CAG repeat determination

PCR of tail snips was performed to determine genotype and CAG repeat count

View evidence from paper

“Genotyping and CAG repeat count were determined by Laragen Inc. (Culver City, CA), from PCR of tail snips taken at 10–15 days of age”