Rearing and Climbing Assessment
behavioralmouseC57BL/6J background with zQ175 CAG repeat expansion
Objective: Measurement of vertical exploratory behavior and climbing activity in light and dark phases across different ages in zQ175 knock-in mice
Protocol Steps
1
Animal identification and genotyping
Mice were ear notched at 10-15 days of age for identification purposes
View evidence from paper
“Animals were ear notched at around 10–15 days”
2
RFID chip implantation
Mice were implanted with RFID electronic chips for tracking purposes
View evidence from paper
“Mice were weaned and implanted with RFID electronic chips (DataMars, OH) for identification at around 21 days”
3
Genotyping and CAG repeat determination
PCR of tail snips was performed to determine genotype and CAG repeat count
View evidence from paper
“Genotyping and CAG repeat count were determined by Laragen Inc. (Culver City, CA), from PCR of tail snips taken at 10–15 days of age”
4
Rearing and climbing assessment in light phase
Measurement of vertical exploratory behavior and climbing activity during light phase
View evidence from paper
“Rearing/climbing in the light phase”
5
Rearing and climbing assessment in dark phase
Measurement of vertical exploratory behavior and climbing activity during dark phase
View evidence from paper
“Rearing/climbing in the dark phase”