Source Paper
Propranolol Administration During Morphine Addiction Attenuates Reinstatement of Drug-Aversive Memories Caused by Exposure to Stressful Stimuli
Cánovas-Cabanes A, Teruel-Fernández FJ, Fernández-López L, Martínez-Laorden E, Navarro-Zaragoza J et al.
Pharmaceuticals (Basel) • 2025
Restraint Stress
Objective: To induce physical stress and evaluate its effect on reinstatement of naloxone-paired conditioned place aversion
Protocol Steps
Drug administration timing
One day after extinction phase, administer propranolol or vehicle injection intraperitoneally
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“One day after the extinction phase, mice received an acute intraperitoneal injection”
Wait period
Wait thirty minutes after drug/vehicle injection before stress procedure
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“Then, after thirty minutes, animals were subjected to a restraint stress protocol”
Restraint stress application
Confine animals individually in well-ventilated 50 mL polypropylene conical tubes for 15 minutes
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“individual confinement within well-ventilated 50 mL polypropylene conical tubes for a duration of 15 min”
Physical restriction details
The restraint tube allows adequate breathing while restricting head and limb movement
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“The restraint tube permitted adequate breathing while the head and limb movement were restricted”
Return to home cage
When stress exposure ends, return animals to their home cages
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“When the stress exposure ended, the animals returned to their home cages”
Control group handling
Control group remains undisturbed in home cages for the same time interval
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“A control group remained undisturbed in their home cages for the same time interval”
Reinstatement testing
Perform reinstatement test on all animals following stress procedure
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“Finally, the reinstatement test was displayed for all the animals”