Experiments/Restraint Stress

Source Paper

Propranolol Administration During Morphine Addiction Attenuates Reinstatement of Drug-Aversive Memories Caused by Exposure to Stressful Stimuli

Cánovas-Cabanes A, Teruel-Fernández FJ, Fernández-López L, Martínez-Laorden E, Navarro-Zaragoza J et al.

Pharmaceuticals (Basel) • 2025

PMC

Restraint Stress

behavioralMouseC57BL/6J

Objective: To induce physical stress and evaluate its effect on reinstatement of naloxone-paired conditioned place aversion

This is a Restraint Stress protocol using Mouse as the model organism. The procedure involves 7 procedural steps, 1 equipment items, 3 materials. Extracted from a 2025 paper published in Pharmaceuticals (Basel).

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Replication Essentials

Model and subjects

Mouse • C57BL/6J • Male • Adult • 179

Study window

~15 minutes hands-on

Core workflow

Drug administration timing • Wait period • Restraint stress application

Primary readouts

Reinstatement of conditioned place aversion
Time spent in naloxone-paired chamber
Number of crossings between compartments

Key equipment and reagents

Well-ventilated 50 mL polypropylene conical tubesPropranolol (β-ARs antagonist)

Before You Start

Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.

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Verify the animal model, intervention setup, and collection timepoints against the source paper.
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Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
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Protocol Steps

Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.

Drug administration timing

One day after extinction phase, administer propranolol or vehicle injection intraperitoneally

View evidence from paper

“One day after the extinction phase, mice received an acute intraperitoneal injection”

Wait period

Wait thirty minutes after drug/vehicle injection before stress procedure

View evidence from paper

“Then, after thirty minutes, animals were subjected to a restraint stress protocol”