Resveratrol Treatment in p25 Transgenic Mice
Objective: To assess the neuroprotective effects of resveratrol and SIRT1 activation in p25 transgenic mice, measuring neurodegeneration, learning capacity, and protein acetylation levels
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Equipment1
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Materials2
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Protocol Steps
Resveratrol administration to p25 transgenic mice
Administer resveratrol to inducible p25 transgenic mice to activate SIRT1 pathway
Note: Dosage and route of administration not specified in provided text
View evidence from paper
“In the inducible p25 transgenic mouse, a model of AD and tauopathies, resveratrol reduced neurodegeneration”
Assess learning capacity
Conduct behavioral testing to measure learning impairment in treated p25 transgenic mice
Note: Specific behavioral test not detailed in provided text
View evidence from paper
“prevented learning impairment”
Measure hippocampal neurodegeneration
Evaluate neurodegeneration in the hippocampus following resveratrol treatment
Note: Specific measurement method not detailed in provided text
View evidence from paper
“resveratrol reduced neurodegeneration in the hippocampus”
Measure protein acetylation levels
Assess acetylation of SIRT1 substrates PGC-1alpha and p53 following resveratrol treatment
Note: Specific analytical method not detailed in provided text
View evidence from paper
“decreased the acetylation of the known SIRT1 substrates PGC-1alpha and p53”
SIRT1 lentivirus hippocampal injection
Inject SIRT1 lentivirus directly into the hippocampus of p25 transgenic mice as alternative intervention
Note: Injection volume, coordinates, and viral titer not specified in provided text
View evidence from paper
“injection of SIRT1 lentivirus in the hippocampus of p25 transgenic mice conferred significant protection against neurodegeneration”