Source Paper
Fecal microbiota transplantation protects rotenone-induced Parkinson’s disease mice via suppressing inflammation mediated by the lipopolysaccharide-TLR4 signaling pathway through the microbiota-gut-brain axis
Zhe Zhao, Jingwen Ning, Xiu-qi Bao, Meiyu Shang, Jingwei Ma et al.
Microbiome • 2021
Rota-Rod Test
Objective: Assessment of motor coordination and balance in mice on a rotating rod
This is a Rota-Rod Test protocol using mouse as the model organism. The procedure involves 8 procedural steps, 1 equipment items, 2 materials. Extracted from a 2021 paper published in Microbiome.
Model and subjects
mouse • C57BL/6J • male • 8 weeks • 20-22 g • 45
Study window
~6 week study window
Core workflow
Animal acclimation • Group assignment • Rotenone treatment initiation
Primary readouts
- Motor coordination performance on rotating rod
- Balance maintenance on rotating rod
- Time to fall from rod
Key equipment and reagents
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Protocol Steps
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Animal acclimation
Mice were acclimated under standard laboratory conditions with 12-hour light/dark cycle, temperature maintained at 22 ± 2°C, humidity 50-60%, with ad libitum access to food and water
Note: Humidity maintained at 50-60%
View evidence from paper
“The mice were then acclimatized (12-h light/dark cycle) under standard conditions (temperature 22 ± 2 °C, humidity 50–60%) with ad libitum access to food and water for 7 days”
Group assignment
45 mice randomly assigned into control group (n=15) and model group (n=30)
Note: Random assignment performed
View evidence from paper
“A total of 45 mice were randomly assigned into two groups: the control group ( n = 15) and the model group ( n = 30)”
Rotenone treatment initiation
Model group received daily oral administration of rotenone; control group received vehicle administration
Note: Daily administration
View evidence from paper
“In the beginning 4 weeks, the model group received the oral administration of rotenone every day. Meanwhile, the control group mice were administrated with vehicle”
Secondary group division
After 4 weeks, model group mice randomly divided into Rotenone group (n=15) and FMT group (n=15)
Note: Random division of model group
View evidence from paper
“After 4 weeks, we randomly divided the model group mice into two groups: Rotenone group ( n = 15) and FMT group ( n = 15)”
FMT treatment administration
FMT group received fecal microbiota transplantation once per day; control and rotenone groups received vehicle administration once daily
Note: Once daily administration
View evidence from paper
“During week 5 and 6, the mice in the FMT group were treated with FMT once per day. In the meantime, the control group and the rotenone group mice received vehicle administration once a day”
Body weight monitoring
All mice weighed daily throughout the experimental period
Note: Daily measurements
View evidence from paper
“These mice were daily weighed for 6 weeks”
Rota-Rod test performance
Behavioral test performed to assess motor coordination and balance
Note: n=15 for each group
View evidence from paper
“behavioral tests were performed at week 6”
Animal sacrifice and tissue collection
All mice sacrificed for further analysis
Note: Performed at week 6
View evidence from paper
“All the mice were sacrificed at week 6 for further analysis”