Rotarod Performance Test
Objective: Evaluation of locomotor ability and motor coordination using an accelerating rod protocol
This is a Rotarod Performance Test protocol using mouse as the model organism. The procedure involves 2 procedural steps, 1 equipment items, 6 materials. Extracted from a 2017 paper published in Redox Biology.
Model and subjects
mouse • Gpx4BIKO and Gpx4(f/f) littermates • unknown • 2-4 months at enrollment
Study window
~3 minutes hands-on
Core workflow
Rotarod apparatus setup • Rotarod performance testing
Primary readouts
- Latency to fall (time in seconds before falling from the accelerating rod)
- Locomotor ability index
- Motor coordination performance
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Rotarod apparatus setup
Configure the Rotamex 4/8 rotarod machine with initial speed and acceleration parameters
Note: Initial speed set to 2 rpm with linear acceleration to 40 rpm
View evidence from paper
“The initial speed was set to 2 rpm with a linear acceleration to 40 rpm”
Rotarod performance testing
Place mouse on the accelerating rod and record latency to fall as an index of locomotor ability
Note: Latency to fall is the primary outcome measure
View evidence from paper
“Latency to fall was recorded as an index for locomotor ability at 4 trials per day with a 2–3 min inter-trial rest period”