Rotarod Performance Test
behavioralmouseGpx4BIKO and Gpx4(f/f) littermates
Objective: Evaluation of locomotor ability and motor coordination using an accelerating rod protocol
Materials & Equipment Checklist
7 items1 from ConductScience
Gather these items before starting the experiment. Check off items as you prepare.
Equipment1
Columbus Instruments • Rotamex 4/8
1 item available from ConductScience
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Protocol Steps
1
Rotarod apparatus setup
Configure the Rotamex 4/8 rotarod machine with initial speed and acceleration parameters
Note: Initial speed set to 2 rpm with linear acceleration to 40 rpm
View evidence from paper
“The initial speed was set to 2 rpm with a linear acceleration to 40 rpm”
2
Rotarod performance testing
Place mouse on the accelerating rod and record latency to fall as an index of locomotor ability
4 trials per day with 2-3 min inter-trial rest period
Note: Latency to fall is the primary outcome measure
View evidence from paper
“Latency to fall was recorded as an index for locomotor ability at 4 trials per day with a 2–3 min inter-trial rest period”