Rotarod Test
Objective: Assessment of motor function in mice during treatment, performed monthly to evaluate rotarod performance
Gather these items before starting the experiment. Check off items as you prepare.
Equipment1
Not specified • Not specified • Not specified • Not specified
Software1
GraphPad Software, Inc., La Jolla, CA, USA • Not specified
1 item available from ConductScience
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Protocol Steps
Divide mice into experimental groups
Mice were divided into 8 groups with 4 different treatments (control, rotenone treated, rotenone treated after hemivagotomy, rotenone treated after partial sympathectomy) and two treatment times (2 and 4 months)
Note: Each group contained n=10 mice
View evidence from paper
“mice were divided in 8 groups (n = 10) with 4 different treatments (control, rotenone treated, rotenone treated after hemivagotomy and rotenone treated after partial sympathectomy) and two treatment times (2 and 4 months)”
Perform rotarod testing
Motor function was assessed using the rotarod test performed once a month during the treatment period
Note: Testing was conducted during the treatment period for both 2 and 4 month treatment groups
View evidence from paper
“Motor and gastrointestinal functions were analyzed once a month with the help of the rotarod and 1-hour stool collection tests during treatment”
Sacrifice animals and prepare tissue
After treatment completion, animals were sacrificed and perfused with 4% PFA for immunohistological staining and image analysis
Note: This step occurred after the treatment period ended
View evidence from paper
“After treatment, animals were sacrificed and perfused with 4% PFA for immunohistological staining and image analysis”