Rotarod Test
Objective: Assessment of motor function in mice during treatment, performed monthly to evaluate rotarod performance
This is a Rotarod Test protocol using mouse as the model organism. The procedure involves 3 procedural steps, 1 equipment items. Extracted from a 2012 paper published in Scientific Reports.
Model and subjects
mouse • Not specified • unknown • Not specified • Not specified • 80
Study window
Estimated timing pending
Core workflow
Divide mice into experimental groups • Perform rotarod testing • Sacrifice animals and prepare tissue
Primary readouts
- Rotarod performance
- Motor function assessment
Key equipment and reagents
Verified items
0
Direct vendor links
0
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Protocol Steps
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Divide mice into experimental groups
Mice were divided into 8 groups with 4 different treatments (control, rotenone treated, rotenone treated after hemivagotomy, rotenone treated after partial sympathectomy) and two treatment times (2 and 4 months)
Note: Each group contained n=10 mice
View evidence from paper
“mice were divided in 8 groups (n = 10) with 4 different treatments (control, rotenone treated, rotenone treated after hemivagotomy and rotenone treated after partial sympathectomy) and two treatment times (2 and 4 months)”
Perform rotarod testing
Motor function was assessed using the rotarod test performed once a month during the treatment period
Note: Testing was conducted during the treatment period for both 2 and 4 month treatment groups
View evidence from paper
“Motor and gastrointestinal functions were analyzed once a month with the help of the rotarod and 1-hour stool collection tests during treatment”
Sacrifice animals and prepare tissue
After treatment completion, animals were sacrificed and perfused with 4% PFA for immunohistological staining and image analysis
Note: This step occurred after the treatment period ended
View evidence from paper
“After treatment, animals were sacrificed and perfused with 4% PFA for immunohistological staining and image analysis”