Rotarod Test
behavioralmouseC57BL/6J
Objective: Evaluation of motor coordination and balance using accelerating rotarod in light and dark phases
Materials & Equipment Checklist
2 items1 from ConductScience
Gather these items before starting the experiment. Check off items as you prepare.
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Protocol Steps
1
Animal identification and genotyping
Mice were ear notched at 10-15 days of age for identification purposes
View evidence from paper
“Animals were ear notched at around 10–15 days”
2
RFID chip implantation
Mice were implanted with RFID electronic chips for identification and tracking
View evidence from paper
“Mice were weaned and implanted with RFID electronic chips (DataMars, OH) for identification at around 21 days”
3
CAG repeat genotyping
Genotyping and CAG repeat count were determined from PCR of tail snips
View evidence from paper
“Genotyping and CAG repeat count were determined by Laragen Inc. (Culver City, CA), from PCR of tail snips taken at 10–15 days of age”
4
Rotarod testing - light phase
Motor coordination and balance evaluation during light phase
View evidence from paper
“Rotarod in the light phase”
5
Rotarod testing - dark phase
Motor coordination and balance evaluation during dark phase
View evidence from paper
“Rotarod in the dark phase”