Rotarod Test
Objective: Motor coordination and balance assessment using rotarod apparatus
This is a Rotarod Test protocol using mouse as the model organism. The procedure involves 2 procedural steps, 1 equipment items, 2 materials. Extracted from a 2016 paper published in Molecular Psychiatry.
Model and subjects
mouse • C57BL/6J background • male • 60-90 days • 81
Study window
~30 minutes hands-on
Core workflow
Animal acclimation to testing room • Rotarod test performance
Primary readouts
- Motor coordination performance
- Balance assessment
Key equipment and reagents
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Protocol Steps
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Animal acclimation to testing room
Animals were given habituation time to the behavioral testing room prior to testing
Note: Behavioral phenotyping was performed between 0900h and 1600h
View evidence from paper
“Animals were given 30 min of habituation to the behavioral testing room. Behavioral phenotyping was performed between 0900 h and 1600 h.”
Rotarod test performance
Rotarod test was performed as the first test in the behavioral test battery, from least to most invasive
Note: Test order was: rotarod, elevated plus maze, marble burying test, open field test, sucrose preference test, novelty suppressed feeding and forced swim test. This order was reversed after chronic restraint stress for a bell-shaped stress exposure.
View evidence from paper
“Tests were performed from the least to the most invasive to minimize the influence of prior test history (in order: rotarod, elevated plus maze, marble burying test, open field test, sucrose preference test, novelty suppressed feeding and forced swim test)”