Rotarod Test
Objective: Assessment of motor coordination and balance by measuring ability to remain on a rotating rod
Gather these items before starting the experiment. Check off items as you prepare.
Equipment1
Not specified • Not specified • Not specified • Not specified
1 item available from ConductScience
As an Amazon Associate, we earn from qualifying purchases. Product links help support this free resource.
Protocol Steps
Induce transient middle cerebral artery occlusion (tMCAO) or permanent distal MCAO (dMCAO)
Perform tMCAO or dMCAO in wild-type (WT) and IL-4 knockout (KO) C57BL/6 mice
Note: Two different surgical approaches used in different animal groups
View evidence from paper
“Transient middle cerebral artery occlusion (tMCAO) or permanent distal MCAO (dMCAO) was induced in wild-type (WT) and IL-4 knockout (KO) C57/BL6 mice”
Administer IL-4 or vehicle infusion
In a separate cohort of WT animals, infuse IL-4 (60 ng/d for 7d) or vehicle into the cerebroventricle after tMCAO
Note: This is a separate cohort from the knockout and tMCAO/dMCAO comparison groups
View evidence from paper
“In a separate cohort of WT animals, IL-4 (60 ng/d for 7d) or vehicle was infused into the cerebroventricle after tMCAO”
Perform Rotarod test
Assess motor coordination and balance using the Rotarod test
Note: Part of behavioral outcome assessment battery
View evidence from paper
“Behavioral outcomes were assessed by the Rotarod, corner, foot fault, and Morris water maze tests”