Secondary H. polygyrus bakeri Challenge Infection

“Mice were infected orally by gavage with 200 infective L3 stage larvae”

“adult H. polygyrus were eliminated from infected mice by oral administration of pyrantel pamoate (1 mg/mouse; Columbia Laboratory) at day 14 post-primary infection”

“and > 5 weeks later the mice were challenge-infected with 200 L3 stage larvae by gavage”

“the mice were challenge-infected with 200 L3 stage larvae by gavage”

“At day 9 post challenge infection, mice were sacrificed and parasite burdens were measured. To count worm, intestines were removed, opened longitudinally”

“placed into a metal strainer on top of a 50 ml tube filled with phosphate buffered saline (PBS; Corning Inc.) for 4 h at 37 °C. Parasites dropped through the filter into the tube and were recovered for counting”

“were recovered for counting on a dissecting microscope”

“Parasite eggs were enumerated by floating eggs in feces collected from individual mice on saturated sodium chloride prior to collection, and counting under a microscope”