Seizure Assessment
Objective: Measured the severity of spontaneous and chemically induced seizures in transgenic mice to assess the protective effects of tau reduction on seizure activity
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Protocol Steps
Spontaneous seizure assessment
Monitored and recorded spontaneous seizure activity in hAPP transgenic mice using electroencephalography
Note: Conducted in multiple lines of hAPP mice with and without tau reduction
View evidence from paper
“Tau reduction also reduced the severity of spontaneous and chemically induced seizures in mice overexpressing both Aβ and Fyn.”
Chemically induced seizure assessment
Induced seizures chemically and measured the severity of resulting seizure activity
Note: Chemical induction method not specified in provided text
View evidence from paper
“Tau reduction also reduced the severity of spontaneous and chemically induced seizures in mice overexpressing both Aβ and Fyn.”
Epileptiform activity recording
Used electroencephalography to record spontaneous epileptiform activity patterns in the brain
Note: Examined across multiple lines of hAPP mice
View evidence from paper
“Using electroencephalography to examine network effects, we found that tau reduction prevented spontaneous epileptiform activity in multiple lines of hAPP mice.”
Whole-cell current recording
Recorded whole-cell currents in acute hippocampal slices to measure excitatory and inhibitory currents and NMDA receptor function
Note: Conducted on slices from hAPP mice with and without tau to understand protective mechanisms
View evidence from paper
“We recorded whole-cell currents in acute hippocampal slices from hAPP mice with and without tau. hAPP mice with tau had increased spontaneous and evoked excitatory currents, reduced inhibitory currents, and NMDA receptor dysfunction.”