Seizure Assessment
Objective: Measured the severity of spontaneous and chemically induced seizures in transgenic mice to assess the protective effects of tau reduction on seizure activity
This is a Seizure Assessment protocol using mouse as the model organism. The procedure involves 4 procedural steps, 2 equipment items. Extracted from a 2011 paper published in Journal of Neuroscience.
Model and subjects
mouse • hAPP transgenic mice (multiple lines mentioned) • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Spontaneous seizure assessment • Chemically induced seizure assessment • Epileptiform activity recording
Primary readouts
- Severity of spontaneous seizures
- Severity of chemically induced seizures
- Presence and frequency of spontaneous epileptiform activity
- Spontaneous excitatory currents in hippocampal slices
Key equipment and reagents
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Protocol Steps
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Spontaneous seizure assessment
Monitored and recorded spontaneous seizure activity in hAPP transgenic mice using electroencephalography
Note: Conducted in multiple lines of hAPP mice with and without tau reduction
View evidence from paper
“Tau reduction also reduced the severity of spontaneous and chemically induced seizures in mice overexpressing both Aβ and Fyn.”
Chemically induced seizure assessment
Induced seizures chemically and measured the severity of resulting seizure activity
Note: Chemical induction method not specified in provided text
View evidence from paper
“Tau reduction also reduced the severity of spontaneous and chemically induced seizures in mice overexpressing both Aβ and Fyn.”
Epileptiform activity recording
Used electroencephalography to record spontaneous epileptiform activity patterns in the brain
Note: Examined across multiple lines of hAPP mice
View evidence from paper
“Using electroencephalography to examine network effects, we found that tau reduction prevented spontaneous epileptiform activity in multiple lines of hAPP mice.”
Whole-cell current recording
Recorded whole-cell currents in acute hippocampal slices to measure excitatory and inhibitory currents and NMDA receptor function
Note: Conducted on slices from hAPP mice with and without tau to understand protective mechanisms
View evidence from paper
“We recorded whole-cell currents in acute hippocampal slices from hAPP mice with and without tau. hAPP mice with tau had increased spontaneous and evoked excitatory currents, reduced inhibitory currents, and NMDA receptor dysfunction.”