Seizure Monitoring and Pharmacological Testing
Objective: Observation and characterization of seizures and assessment of response to antiepileptic medications in mice lacking the β3 subunit of the GABA A receptor
This is a Seizure Monitoring and Pharmacological Testing protocol using mouse as the model organism. The procedure involves 3 procedural steps, 1 equipment items. Extracted from a 1998 paper published in Journal of Neuroscience.
Model and subjects
mouse • Not specified in provided text • unknown • Not specified in provided text • Not specified in provided text
Study window
Estimated timing pending
Core workflow
Seizure observation and characterization • Pharmacological testing with antiepileptic medications • Assessment of behavioral characteristics
Primary readouts
- Electroencephalographic abnormalities
- Seizure occurrence and characteristics
- Pharmacological response to antiepileptic medications
- Learning and memory performance
Key equipment and reagents
Verified items
0
Direct vendor links
0
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Protocol Steps
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Seizure observation and characterization
Observe and characterize seizures in mice lacking the β3 subunit of the GABA A receptor
Note: Seizures were observed and their characteristics documented
View evidence from paper
“The seizures that are observed in these mice showed a pharmacological response profile to antiepileptic medications”
Pharmacological testing with antiepileptic medications
Assess response to antiepileptic medications to determine pharmacological response profile
Note: Response profile was compared to that observed in Angelman syndrome patients
View evidence from paper
“The seizures that are observed in these mice showed a pharmacological response profile to antiepileptic medications similar to that observed in AS”
Assessment of behavioral characteristics
Evaluate learning and memory deficits, motor skills, hyperactivity, and rest-activity cycle disturbances
Note: Features assessed were common to Angelman syndrome
View evidence from paper
“Additionally, these mice exhibited learning and memory deficits, poor motor skills on a repetitive task, hyperactivity, and a disturbed rest–activity cycle”