Source Paper
Source Paper
Hang Fang, Lisi Huang, Ian Welch, Chris Norley, David W. Holdsworth et al.
Scientific Reports • 2018
Objective: Sham surgical control procedure performed on mice to serve as comparison for DMM (destabilization of medial meniscus) surgery effects on osteoarthritis development
This is a Sham Surgery Control protocol using mouse as the model organism. The procedure involves 10 procedural steps, 2 equipment items, 4 materials. Extracted from a 2018 paper published in Scientific Reports.
Model and subjects
mouse • C57/Bl6 wild type • unknown • 12 weeks at time of surgery • not specified • 18
Study window
~12 week study window | ~8 hours hands-on
Core workflow
Animal housing and acclimation • Surgical procedure on left knee joint • Antibiotic administration
Primary readouts
Key equipment and reagents
Verified items
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Direct vendor links
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House all mice on a 12-hour light/dark cycle with unrestricted access to standard mouse food and water prior to surgery
Note: Mice were 12 weeks of age at time of surgery
“All mice were housed on a 12-hour light/dark cycle with unrestricted access to standard mouse food and water”
Perform sham surgery on the left knee joint of mice as previously described in references 20 and 21
Note: Sham surgery serves as control comparison to DMM surgery. Specific surgical details referenced in citations 20 and 21
“Surgery was performed on the left knee joint of mice as previously described 20, 21”
Inject Amoxicillin subcutaneously immediately after surgery to prevent joint infection
Note: Single dose administration
“A single dose of Amoxicillin (Novopharm, Toronto, Ontario, Canada) was injected subcutaneously right after the surgery to prevent joint infection (20 mg/kg)”
Administer Buprenorphine subcutaneously at 0 hours post-surgery to minimize pain
Note: First of two doses
“Buprenorphine (Schering-Plough, Herfordshire, UK) was administered twice (0 and 4 hours after surgery) subcutaneously to minimized pain (0.05 mg/kg)”
Administer Buprenorphine subcutaneously at 4 hours post-surgery to minimize pain
Note: Second of two doses
“Buprenorphine (Schering-Plough, Herfordshire, UK) was administered twice (0 and 4 hours after surgery) subcutaneously to minimized pain (0.05 mg/kg)”
House mice in colony cages with up to 6 mice per cage after surgery
Note: Mice remain in colony housing throughout recovery and experimental period
“Mice were housed in colony cage after the surgery (up to 6 mice in one cage)”
Place running wheels in cages to encourage exercise and physical activity
Note: Wheels added 2 days after surgery to allow initial recovery period
“running wheels were put in two days after the surgery to encourage exercise”
Terminate mice and collect tissues at 2 weeks post-surgery
Note: SHAM group at 2 weeks: 5 mice
“three termination time-points (2, 5 and 10 weeks post-surgery)”
Terminate mice and collect tissues at 5 weeks post-surgery
Note: SHAM group at 5 weeks: 7 mice
“three termination time-points (2, 5 and 10 weeks post-surgery)”
Terminate mice and collect tissues at 10 weeks post-surgery
Note: SHAM group at 10 weeks: 6 mice
“three termination time-points (2, 5 and 10 weeks post-surgery)”
This section explains what the experiment is doing, which readouts matter, what the data artifacts usually look like, and how the analysis should flow from raw capture to reported result.
Sham surgical control procedure performed on mice to serve as comparison for DMM (destabilization of medial meniscus) surgery effects on osteoarthritis development
Objective
Sham surgical control procedure performed on mice to serve as comparison for DMM (destabilization of medial meniscus) surgery effects on osteoarthritis development
Subjects
From papermouse • C57/Bl6 wild type • unknown • 12 weeks at time of surgery • not specified
Sample count
From paper18
Cohort notes
From paperRandomly designated into SHAM group with three termination time-points (2, 5, and 10 weeks post-surgery).
Animal housing and acclimation (ongoing until surgery at 12 weeks of age)
Surgical procedure on left knee joint (not specified)
Antibiotic administration (immediately post-surgery)
Analgesic administration - first dose (0 hours post-surgery)
Tissue samples from left knee joint at specified time-points
From papernot specified in methods section
Artifact type
Endpoint measurements summarized by group or timepoint
Comparison focus
Compare endpoint magnitude between groups, timepoints, or both
Comparison of sham surgery effects versus DMM surgery effects on osteoarthritis development
From papernot specified in methods section
Artifact type
Endpoint measurements summarized by group or timepoint
Comparison focus
Compare endpoint magnitude between groups, timepoints, or both
Tissue samples from left knee joint at specified time-points
From paperRaw artifact
Per-sample or per-animal endpoint measurements collected during the experiment
Processed artifact
Structured table with cleaned measurements ready for comparison
Final reported form
Summary statistics and between-group or across-timepoint comparisons
Comparison of sham surgery effects versus DMM surgery effects on osteoarthritis development
From paperRaw artifact
Per-sample or per-animal endpoint measurements collected during the experiment
Processed artifact
Structured table with cleaned measurements ready for comparison
Final reported form
Summary statistics and between-group or across-timepoint comparisons
Acquisition
Collect raw experimental outputs with enough metadata to preserve sample identity, condition, and timing.
Preprocessing / cleaning
not specified in methods section
Scoring or quantification
Quantify the primary readouts for this experiment: Tissue samples from left knee joint at specified time-points; Comparison of sham surgery effects versus DMM surgery effects on osteoarthritis development.
Statistical comparison
Statistical method not yet structured for this page.
Reporting output
Report representative outputs alongside summary comparisons for Tissue samples from left knee joint at specified time-points, Comparison of sham surgery effects versus DMM surgery effects on osteoarthritis development.
Source links and direct wording from the methods section for validation and deeper review.
Citation
Hang Fang et al. (2018). Early Changes of Articular Cartilage and Subchondral Bone in The DMM Mouse Model of Osteoarthritis. Scientific Reports
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not specified
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Novopharm, Toronto, Ontario, Canada
Schering-Plough, Herfordshire, UK
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not specified
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Current status surfaces were computed from experiment data updated Feb 28, 2026.
Source access
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Steps
10
Evidence Quotes
16
Protocol Items
6
Linked Products
0
Canonical Sync
Pending
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Computed from the current experiment record updated Feb 28, 2026.
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Steps
10
Evidence
16
Specific Products
0/0
Canonical Sync
Pending
What this score means
The verification score reflects evidence coverage, subject detail, paper provenance, step depth, and whether linked products resolve to specific item pages instead of generic searches.
Computed from the current experiment record updated Feb 28, 2026.
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