Sham Surgery Control
Objective: Sham surgical control procedure performed on mice to serve as comparison for DMM (destabilization of medial meniscus) surgery effects on osteoarthritis development
Gather these items before starting the experiment. Check off items as you prepare.
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Protocol Steps
Animal housing and acclimation
House all mice on a 12-hour light/dark cycle with unrestricted access to standard mouse food and water prior to surgery
Note: Mice were 12 weeks of age at time of surgery
View evidence from paper
“All mice were housed on a 12-hour light/dark cycle with unrestricted access to standard mouse food and water”
Surgical procedure on left knee joint
Perform sham surgery on the left knee joint of mice as previously described in references 20 and 21
Note: Sham surgery serves as control comparison to DMM surgery. Specific surgical details referenced in citations 20 and 21
View evidence from paper
“Surgery was performed on the left knee joint of mice as previously described 20, 21”
Antibiotic administration
Inject Amoxicillin subcutaneously immediately after surgery to prevent joint infection
Note: Single dose administration
View evidence from paper
“A single dose of Amoxicillin (Novopharm, Toronto, Ontario, Canada) was injected subcutaneously right after the surgery to prevent joint infection (20 mg/kg)”
Analgesic administration - first dose
Administer Buprenorphine subcutaneously at 0 hours post-surgery to minimize pain
Note: First of two doses
View evidence from paper
“Buprenorphine (Schering-Plough, Herfordshire, UK) was administered twice (0 and 4 hours after surgery) subcutaneously to minimized pain (0.05 mg/kg)”
Analgesic administration - second dose
Administer Buprenorphine subcutaneously at 4 hours post-surgery to minimize pain
Note: Second of two doses
View evidence from paper
“Buprenorphine (Schering-Plough, Herfordshire, UK) was administered twice (0 and 4 hours after surgery) subcutaneously to minimized pain (0.05 mg/kg)”
Post-operative housing
House mice in colony cages with up to 6 mice per cage after surgery
Note: Mice remain in colony housing throughout recovery and experimental period
View evidence from paper
“Mice were housed in colony cage after the surgery (up to 6 mice in one cage)”
Running wheel placement
Place running wheels in cages to encourage exercise and physical activity
Note: Wheels added 2 days after surgery to allow initial recovery period
View evidence from paper
“running wheels were put in two days after the surgery to encourage exercise”
Tissue collection - 2 week time-point
Terminate mice and collect tissues at 2 weeks post-surgery
Note: SHAM group at 2 weeks: 5 mice
View evidence from paper
“three termination time-points (2, 5 and 10 weeks post-surgery)”
Tissue collection - 5 week time-point
Terminate mice and collect tissues at 5 weeks post-surgery
Note: SHAM group at 5 weeks: 7 mice
View evidence from paper
“three termination time-points (2, 5 and 10 weeks post-surgery)”
Tissue collection - 10 week time-point
Terminate mice and collect tissues at 10 weeks post-surgery
Note: SHAM group at 10 weeks: 6 mice
View evidence from paper
“three termination time-points (2, 5 and 10 weeks post-surgery)”