SHIRPA Protocol
Objective: Phenotype assessment to evaluate neurological and behavioral characteristics in mice, specifically assessing gait, motor function, and neurological status in a Refsum disease mouse model
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Equipment2
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Protocol Steps
Phenotype Assessment
Conduct standardized phenotype assessment using the SHIRPA protocol to evaluate neurological and behavioral characteristics
Note: Protocol evaluates neurological status and behavioral characteristics
View evidence from paper
“Phenotype assessment using the SHIRPA protocol and subsequent automated gait analysis using the CatWalk system revealed unsteady gait”
Automated Gait Analysis
Perform automated gait analysis using the CatWalk system to measure paw print area and base of support
Note: Measures paw print area for both fore- and hindpaws and base of support for hindpaws
View evidence from paper
“automated gait analysis using the CatWalk system revealed unsteady gait with strongly reduced paw print area for both fore- and hindpaws and reduced base of support for the hindpaws”
Motor Nerve Conduction Velocity Measurements
Measure motor nerve conduction velocity to assess peripheral neuropathy
Note: Reveals presence of peripheral neuropathy
View evidence from paper
“Motor nerve conduction velocity measurements revealed a peripheral neuropathy”