SHIRPA Protocol
Objective: Phenotype assessment to evaluate neurological and behavioral characteristics in mice, specifically assessing gait, motor function, and neurological status in a Refsum disease mouse model
This is a SHIRPA Protocol protocol using mouse as the model organism. The procedure involves 3 procedural steps, 2 equipment items. Extracted from a 2008 paper published in Proceedings of the National Academy of Sciences.
Model and subjects
mouse • Phyh knockout • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Phenotype Assessment • Automated Gait Analysis • Motor Nerve Conduction Velocity Measurements
Primary readouts
- Gait characteristics (unsteady gait)
- Paw print area for forepaws
- Paw print area for hindpaws
- Base of support for hindpaws
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Phenotype Assessment
Conduct standardized phenotype assessment using the SHIRPA protocol to evaluate neurological and behavioral characteristics
Note: Protocol evaluates neurological status and behavioral characteristics
View evidence from paper
“Phenotype assessment using the SHIRPA protocol and subsequent automated gait analysis using the CatWalk system revealed unsteady gait”
Automated Gait Analysis
Perform automated gait analysis using the CatWalk system to measure paw print area and base of support
Note: Measures paw print area for both fore- and hindpaws and base of support for hindpaws
View evidence from paper
“automated gait analysis using the CatWalk system revealed unsteady gait with strongly reduced paw print area for both fore- and hindpaws and reduced base of support for the hindpaws”
Motor Nerve Conduction Velocity Measurements
Measure motor nerve conduction velocity to assess peripheral neuropathy
Note: Reveals presence of peripheral neuropathy
View evidence from paper
“Motor nerve conduction velocity measurements revealed a peripheral neuropathy”