Source Paper
Hydrogen‐Mediated Activation of the Nrf2/HO‐1 Signaling Pathway Improves Cognitive Impairment in Sleep‐Deprived Mice
Xiao Q, Zhou S, Tang B, Zhu Y
CNS Neurosci Ther • 2026
Sleep Deprivation Model
Objective: To generate a sleep deprivation model in mice using a modified multiple-platform technique to study sleep deprivation effects
This is a Sleep Deprivation Model protocol using Mouse as the model organism. The procedure involves 6 procedural steps, 1 equipment items, 4 materials. Extracted from a 2026 paper published in CNS Neurosci Ther.
Model and subjects
Mouse • C57BL/6 • Male and female • 6 weeks old • 60
Study window
Estimated timing pending
Core workflow
Animal habituation • Group assignment • Platform setup
Primary readouts
- Morris Water Maze Test
- Novel Object Recognition Test
- Histological analysis (H&E staining)
- Nissl staining
Key equipment and reagents
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Protocol Steps
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Animal habituation
Maintain mice in regulated environment and allow habituation period
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“Animals were habituated for 1 week prior to experiments”
Group assignment
Randomly divide mice into experimental groups
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“Sixty mice were randomly divided into six groups (n = 10 per group, sexes balanced)”
Platform setup
Place platforms in cages and fill with water to specified depth
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“containing 12 platforms (3 cm diameter, 5 cm height) submerged in water to a depth 1 cm below”
Sleep deprivation protocol
Subject mice to sleep deprivation using the platform method for specified duration
View evidence from paper
“sleep deprivation using the modified multiple platform method (18 h/day for 28 consecutive days)”
Animal movement
Allow animals to move freely between platforms
View evidence from paper
“Animals could move freely by jumping among platforms”
Continuous access provision
Ensure continuous access to food and water throughout the protocol
View evidence from paper
“Food and water were continuously accessible”