Source Paper
Hydrogen‐Mediated Activation of the Nrf2/HO‐1 Signaling Pathway Improves Cognitive Impairment in Sleep‐Deprived Mice
Xiao Q, Zhou S, Tang B, Zhu Y
CNS Neurosci Ther • 2026
Sleep Deprivation Model
behavioralMouseC57BL/6
Objective: To generate a sleep deprivation model in mice using a modified multiple-platform technique to study sleep deprivation effects
Protocol Steps
1
Animal habituation
Maintain mice in regulated environment and allow habituation period
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“Animals were habituated for 1 week prior to experiments”
2
Group assignment
Randomly divide mice into experimental groups
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“Sixty mice were randomly divided into six groups (n = 10 per group, sexes balanced)”
3
Platform setup
Place platforms in cages and fill with water to specified depth
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“containing 12 platforms (3 cm diameter, 5 cm height) submerged in water to a depth 1 cm below”
4
Sleep deprivation protocol
Subject mice to sleep deprivation using the platform method for specified duration
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“sleep deprivation using the modified multiple platform method (18 h/day for 28 consecutive days)”
5
Animal movement
Allow animals to move freely between platforms
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“Animals could move freely by jumping among platforms”
6
Continuous access provision
Ensure continuous access to food and water throughout the protocol
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“Food and water were continuously accessible”