Experiments/Social Interaction Test

Source Paper

Age-related changes in behavior in C57BL/6J mice from young adulthood to middle age

Hirotaka Shoji, Keizo Takao, Satoko Hattori, Tsuyoshi Miyakawa

Molecular Brain • 2016

DOI PMC

Social Interaction Test

behavioralmouseC57BL/6J and genetically engineered mice with C57BL/6J genetic background

Objective: Measurement of social behavior and interaction between mice

This is a Social Interaction Test protocol using mouse as the model organism. The procedure involves 5 procedural steps, 2 equipment items, 2 materials. Extracted from a 2016 paper published in Molecular Brain.

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Replication Essentials

Model and subjects

mouse • C57BL/6J and genetically engineered mice with C57BL/6J genetic background • male • Not specified • Not specified • 1739

Study window

~1 day study window

Core workflow

Animal transport and acclimation • Housing conditions • Test battery order

Primary readouts

Social behavior measurements
Social interaction between mice

Key equipment and reagents

Plastic cagesSocial interaction testing apparatus

Before You Start

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Protocol Steps

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Animal transport and acclimation

Genetically engineered mice and wild-type control mice were transported from animal facilities of other laboratories or vendors to the laboratory

Not specifiedNot specified

Note: Mice were transported from external sources

View evidence from paper

“Genetically engineered mice and their wild-type control mice were transported from the animal facilities of other laboratories or vendors to our laboratory”

Housing conditions

Mice were housed in plastic cages with sterilized PaperClean Bedding under a 12-hour light/dark cycle with lights on at 7:00 am, with ad libitum access to food and water

Not specifiedNot specified

Note: Housing density varied: 1.3% with 1 animal, 2.3% with 2 animals, 6.7% with 3 animals, 80.1% with 4 animals, 8.9% with 5 animals, and 0.7% with 6 animals per cage at the beginning of test battery

View evidence from paper

“housed in plastic cages with sterilized PaperClean Bedding (Japan SLC) under a 12-hr light/dark cycle (lights on at 7:00 am) with access to food (CRF-1, Oriental Yeast Co., Ltd.) and water ad libitum”

Test battery order

Mice were subjected to a behavioral test battery in a standardized order. Social interaction test was performed after elevated plus maze and hot plate tests, and before rotarod test

Not specifiedNot specified

Note: Behavioral testing was performed between 9:00 a.m. and 6:00 p.m. Interval between tests was at least 1 day. More than 75% of mice were tested in accordance with the standard order, though some strains had tests omitted or reordered

View evidence from paper

“The mice were generally tested in the following order; general health and neurological screening, light/dark transition, open field, elevated plus maze, hot plate, social interaction, rotarod, startle response/prepulse inhibition, Porsolt forced swim, Barnes maze, contextual and cued fear conditioning, and tail suspension tests. The interval between tests was at least 1 day.”