Source Paper
Age-related changes in behavior in C57BL/6J mice from young adulthood to middle age
Hirotaka Shoji, Keizo Takao, Satoko Hattori, Tsuyoshi Miyakawa
Molecular Brain • 2016
Social Interaction Test
Objective: Measurement of social behavior and interaction between mice
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Equipment2
Materials2
Software1
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Protocol Steps
Animal transport and acclimation
Genetically engineered mice and wild-type control mice were transported from animal facilities of other laboratories or vendors to the laboratory
Note: Mice were transported from external sources
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“Genetically engineered mice and their wild-type control mice were transported from the animal facilities of other laboratories or vendors to our laboratory”
Housing conditions
Mice were housed in plastic cages with sterilized PaperClean Bedding under a 12-hour light/dark cycle with lights on at 7:00 am, with ad libitum access to food and water
Note: Housing density varied: 1.3% with 1 animal, 2.3% with 2 animals, 6.7% with 3 animals, 80.1% with 4 animals, 8.9% with 5 animals, and 0.7% with 6 animals per cage at the beginning of test battery
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“housed in plastic cages with sterilized PaperClean Bedding (Japan SLC) under a 12-hr light/dark cycle (lights on at 7:00 am) with access to food (CRF-1, Oriental Yeast Co., Ltd.) and water ad libitum”
Test battery order
Mice were subjected to a behavioral test battery in a standardized order. Social interaction test was performed after elevated plus maze and hot plate tests, and before rotarod test
Note: Behavioral testing was performed between 9:00 a.m. and 6:00 p.m. Interval between tests was at least 1 day. More than 75% of mice were tested in accordance with the standard order, though some strains had tests omitted or reordered
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“The mice were generally tested in the following order; general health and neurological screening, light/dark transition, open field, elevated plus maze, hot plate, social interaction, rotarod, startle response/prepulse inhibition, Porsolt forced swim, Barnes maze, contextual and cued fear conditioning, and tail suspension tests. The interval between tests was at least 1 day.”
Social interaction test
Social interaction test was performed as part of the behavioral test battery to measure social behavior and interaction between mice
Note: Specific details about the social interaction test procedure are not provided in this methods section
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“social interaction”
Apparatus cleaning
After all tests, apparatus were cleaned with super hypochlorous water and 70% ethanol to prevent bias due to olfactory cues
Note: Cleaning was performed between tests to eliminate olfactory cues
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“After the tests, all apparatus were cleaned with super hypochlorous water and 70% ethanol to prevent a bias due to olfactory cues”