Social Interaction Test
Objective: Assessment of social interaction behavior in offspring to measure deficits associated with prenatal immune challenge
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Protocol Steps
Prenatal Immune Challenge Administration
Pregnant dams receive intravenous injection of Poly-I:C on gestation day 17
Note: Administered on late gestation day (GD) 17
View evidence from paper
“prenatal exposure to the viral mimic polyriboinosinic-polyribocytidilic acid (Poly-I:C; 5 mg/kg, i.v.) on gestation day (GD) 17”
Social Interaction Testing
Assessment of social interaction behavior in offspring born to immune-challenged mothers
Note: Conducted in both male and female offspring
View evidence from paper
“prenatal exposure to the viral mimic polyriboinosinic-polyribocytidilic acid (Poly-I:C; 5 mg/kg, i.v.) on gestation day (GD) 17 led to significant deficits in social interaction”
Anhedonia Assessment
Measurement of anhedonic behavior in offspring
Note: Conducted in both male and female offspring
View evidence from paper
“led to significant deficits in social interaction, anhedonic behavior, and alterations in the locomotor”
Apomorphine Treatment and Behavioral Response Testing
Acute apomorphine treatment followed by assessment of locomotor and stereotyped behavioral responses
Note: Conducted in both male and female offspring
View evidence from paper
“alterations in the locomotor and stereotyped behavioral responses to acute apomorphine (APO) treatment in both male and female offspring”
Latent Inhibition Testing
Assessment of behavioral/cognitive flexibility indexed by latent inhibition effect
Note: Abnormally enhanced latent inhibition effect observed in male but not female offspring
View evidence from paper
“male but not female offspring born to immune challenged mothers displayed behavioral/cognitive inflexibility as indexed by the presence of an abnormally enhanced latent inhibition (LI) effect”