Spared Nerve Injury (SNI)
Objective: To investigate the impact of spared nerve injury (SNI) neuropathic pain in mice on hippocampal-dependent behavior and underlying cellular and molecular changes
This is a Spared Nerve Injury (SNI) protocol using mouse as the model organism. The procedure involves 6 procedural steps. Extracted from a 2012 paper published in Journal of Neuroscience.
Model and subjects
mouse • Not specified in provided text • unknown • Not specified in provided text • Not specified in provided text
Study window
Estimated timing pending
Core workflow
Spared Nerve Injury (SNI) Surgery • Contextual Fear Extinction Testing • Anxiety-like Behavior Assessment
Primary readouts
- Contextual fear extinction ability
- Anxiety-like behavior
- Extracellular signal-regulated kinase (ERK) expression and phosphorylation
- Neurogenesis in hippocampus
Key equipment and reagents
Verified items
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Direct vendor links
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Protocol Steps
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Spared Nerve Injury (SNI) Surgery
Surgical induction of neuropathic pain through spared nerve injury procedure
Note: SNI animals were compared with Sham control animals
View evidence from paper
“we investigated the impact of spared nerve injury (SNI) neuropathic pain in mice on hippocampal-dependent behavior”
Contextual Fear Extinction Testing
Assessment of hippocampal-dependent fear extinction behavior in SNI and Sham animals
Note: SNI animals were unable to extinguish contextual fear compared to controls
View evidence from paper
“SNI animals were unable to extinguish contextual fear and showed increased anxiety-like behavior”
Anxiety-like Behavior Assessment
Measurement of anxiety-like behavior in SNI compared with Sham animals
Note: SNI mice showed increased anxiety-like behavior
View evidence from paper
“SNI animals were unable to extinguish contextual fear and showed increased anxiety-like behavior”
Hippocampal Extracellular Signal-Regulated Kinase (ERK) Analysis
Measurement of reduced extracellular signal-regulated kinase expression and phosphorylation in hippocampus
Note: SNI mice exhibited reduced ERK expression and phosphorylation compared to Sham animals
View evidence from paper
“SNI mice compared with Sham animals exhibited hippocampal (1) reduced extracellular signal-regulated kinase expression and phosphorylation”
Neurogenesis Assessment
Measurement of decreased neurogenesis in hippocampus of SNI animals
Note: SNI mice showed decreased neurogenesis compared to Sham controls
View evidence from paper
“SNI mice compared with Sham animals exhibited hippocampal (2) decreased neurogenesis”
Short-term Synaptic Plasticity Evaluation
Assessment of altered short-term synaptic plasticity in hippocampus of SNI animals
Note: SNI mice exhibited altered short-term synaptic plasticity
View evidence from paper
“SNI mice compared with Sham animals exhibited hippocampal (3) altered short-term synaptic plasticity”