Spatial Learning Test
Objective: Assessing spatial learning performance as a control measure in mice from enriched and standard housing conditions
This is a Spatial Learning Test protocol using mouse as the model organism. The procedure involves 5 procedural steps, 1 materials. Extracted from a 2002 paper published in Journal of Neuroscience.
Model and subjects
mouse • Not specified • Not specified • adult • Not specified • Not specified
Study window
~5.7 week study window | ~4 hours hands-on
Core workflow
Housing condition assignment • BrdU injection • Early timepoint assessment
Primary readouts
- Number of BrdU-labeled neurons at 4 hours post-injection
- Number of surviving progenitors at 3 weeks post-injection
- Spatial learning performance
- Olfactory memory performance
Key equipment and reagents
Verified items
0
Direct vendor links
0
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Housing condition assignment
House animals in either standard or odor-enriched environment
Note: Two experimental groups: standard housing and odor-enriched housing
View evidence from paper
“Animals housed in either a standard or an odor-enriched environment for 40 d”
BrdU injection
Inject animals intraperitoneally with bromodeoxyuridine to detect proliferation among progenitor cells
Note: Used to follow survival of progenitor cells in the main olfactory bulb
View evidence from paper
“injected intraperitoneally with bromodeoxyuridine (BrdU) to detect proliferation among progenitor cells and to follow their survival in the MOB”
Early timepoint assessment
Assess number of BrdU-labeled neurons at 4 hours after BrdU injection
Note: No alteration in number of BrdU-labeled neurons observed at this timepoint
View evidence from paper
“The number of BrdU-labeled neurons was not altered 4 hr after a single BrdU injection”
Late timepoint assessment
Assess number of surviving progenitors in the main olfactory bulb
Note: Number of surviving progenitors was markedly increased in animals housed in enriched environment
View evidence from paper
“the number of surviving progenitors 3 weeks after BrdU injection was markedly increased in animals housed in an enriched environment”
Spatial learning assessment
Test spatial learning performance as a control measure
Note: No change in spatial learning performance observed between enriched and standard housing groups
View evidence from paper
“adult mice housed in odor-enriched cages display improved olfactory memory without a change in spatial learning performance”