Spatial Memory Testing
Objective: Assessment of intact spatial memory in hippocampal-injured animals to evaluate behavioral recovery following neurotoxin insult
This is a Spatial Memory Testing protocol using mouse as the model organism. The procedure involves 4 procedural steps, 2 equipment items, 2 materials. Extracted from a 2001 paper published in Journal of Neuroscience.
Model and subjects
mouse • Not specified • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Treadmill exercise protocol • Hippocampal injury induction • Spatial memory assessment
Primary readouts
- Spatial memory performance in hippocampal-injured mice
- Recovery of behavioral performance compared to sedentary controls
- Neuronal impairment or loss assessment
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Treadmill exercise protocol
Laboratory rodents were submitted to treadmill running as either a preventive measure before neurotoxin insult or as a therapeutic intervention after insult
Note: Exercise was administered either before or after neurotoxin insult of the hippocampus
View evidence from paper
“Laboratory rodents were submitted to treadmill running (1 km/d) either before or after neurotoxin insult of the hippocampus”
Hippocampal injury induction
Domoic acid neurotoxin was administered to induce hippocampal insult in experimental animals
Note: Timing relative to exercise intervention varied (before or after)
View evidence from paper
“neurotoxin insult of the hippocampus (domoic acid)”
Spatial memory assessment
Behavioral testing was conducted to assess spatial memory performance in hippocampal-injured animals
Note: Testing compared exercising animals versus sedentary controls
View evidence from paper
“animals show recovery of behavioral performance compared with sedentary ones, i.e., intact spatial memory in hippocampal-injured mice”
Anti-IGF-I antibody administration
Blocking anti-IGF-I antibody was administered subcutaneously to exercising animals to inhibit exercise-induced brain uptake of IGF-I
Note: This intervention was used to determine if neuroprotection by exercise is mediated by IGF-I
View evidence from paper
“subcutaneous administration of a blocking anti-IGF-I antibody to exercising animals to inhibit exercise-induced brain uptake of IGF-I”