Spatial Memory Testing
Objective: Assessment of intact spatial memory in hippocampal-injured animals to evaluate behavioral recovery following neurotoxin insult
Gather these items before starting the experiment. Check off items as you prepare.
Equipment2
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Protocol Steps
Treadmill exercise protocol
Laboratory rodents were submitted to treadmill running as either a preventive measure before neurotoxin insult or as a therapeutic intervention after insult
Note: Exercise was administered either before or after neurotoxin insult of the hippocampus
View evidence from paper
“Laboratory rodents were submitted to treadmill running (1 km/d) either before or after neurotoxin insult of the hippocampus”
Hippocampal injury induction
Domoic acid neurotoxin was administered to induce hippocampal insult in experimental animals
Note: Timing relative to exercise intervention varied (before or after)
View evidence from paper
“neurotoxin insult of the hippocampus (domoic acid)”
Spatial memory assessment
Behavioral testing was conducted to assess spatial memory performance in hippocampal-injured animals
Note: Testing compared exercising animals versus sedentary controls
View evidence from paper
“animals show recovery of behavioral performance compared with sedentary ones, i.e., intact spatial memory in hippocampal-injured mice”
Anti-IGF-I antibody administration
Blocking anti-IGF-I antibody was administered subcutaneously to exercising animals to inhibit exercise-induced brain uptake of IGF-I
Note: This intervention was used to determine if neuroprotection by exercise is mediated by IGF-I
View evidence from paper
“subcutaneous administration of a blocking anti-IGF-I antibody to exercising animals to inhibit exercise-induced brain uptake of IGF-I”